摘要
目的探讨复发性流产易感性与人类白细胞抗原(HLA)DR、DQ区域基因多态性的关系。方法采用序列特异性引物聚合酶链反应(PCR-SSP),分析200例复发性流产患者(患者组)和200例无不良妊娠史正常妇女(对照组)的DRB1和DQB1基因型。结果患者组中的DQB1*03(39.25%)等位基因频率显著高于对照组(32.5%)(P=0.047<0.05,RR=1.208),DQB1*05(14%)等位基因频率较对照组显著降低(22.75%)(P=0.001<0.05,RR=0.615);患者组中DRB1*09(14%)等位基因频率显著高于对照组(9.25%)(P=0.036<0.05,RR=1.514),DRB1*12(8.5%)等位基因频率较对照组显著降低(14%)(P=0.014<0.05,RR=0.607)。结论河南地区汉族人群中DQB1*03、DRB1*09可能是复发性流产的易感基因,而DQB1*05、DRB1*12可能对复发性流产的发生有保护作用。
Objective： To investigate whether human leucocyte antigen DRB 1, DQB1 （HLA -DRB1, DQB1 ） genens polymorphism are related to the susceptibility to recurrent spontaneous abortion （RSA） patients. Methods： HLA -DRBI and HLA -DQB1 gene typings are performed by Polymerase chain reaction - specific sequence primers （ PCR - SSP） method in 200 cases of recurrent spontaneous abortion patients and 200 women with normal pregnancy history. Results： In recurrent spontaneous abortion group, the frequency of DQB1 ＊ 03 （39. 25 % ） allele is increase significantly compared with that of controls （32. 5% ） （P = 0. 047 〈 0.05, RR = 1. 208） and the frequency of DQB1 ＊ 05 （ 14% ） allele is decreased significantly （22. 75% ） （P = 0. 001 〈 O. 05, RR = 0. 615） ; also in RSA group, the frequency of DRB1 ＊ 09 （ 14% ） allele is increased significantly compared with that of controls （9. 25% ） （P =0. 036 〈0. 05, RR = 1. 514） and the frequency of DRB1 ＊ 12 （8.5%） allele is decreased significantly （ 14% ） （P = 0. 014 〈 0. 05, RR =0. 607）. Conclusion： The results suggest that DQB1 ＊ 03 and DRB1 ＊ 09 allele may be a susceptible gene of recurrent spontaneous abortion, while DQB1 ＊ 05 and DRB1 ＊ 12 allele may be a protective factor for recurrent spontaneous abortion.
出处
《中国优生与遗传杂志》
2011年第12期30-32,共3页
Chinese Journal of Birth Health & Heredity
关键词
复发性流产
人类白细胞抗原
等位基因频率
多态性
Recurrent spontaneous abortion
Human leucocyte antigen
Allele frequency
Polymorphism
