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桑皱褶螺原体在桑树体内的分布、生化反应及理化处理效应的研究 被引量:3

Distribution of Spiroplasma in Host plant,Biochemieal Response,Effects of Physical and Chemical Treatment
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摘要 桑皱褶螺原体(Spiroplasma sp)分布于桑叶及枝、根等部位的韧皮部中。根部为该病原体的越冬部位。桑感染螺原体后,氧化酶、过氧化物酶的活性,多元酚、纤维素及木质素等形成发生变化。其中抗病品种毛桑的氧化酶活性比感病品种桐乡青强,过氧化物酶的活性则相反。用比色法结合紫外吸收测定多元酚的积累浓度可达100ppm 以上。病枝木质部所含的纤维素及木质素比健枝的显著为少,因此而引起病树的柔枝症。用50~55℃温汤、500ppm 盐酸土霉素浸渍及微波处理病枝等,对皱褶螺原体有抑制作用。 The mulberry crinkle pathogen(Spiroplasma sp.)thrives systematically in phloem of leaf, branch and root of host plants and overwinters in the roots.The biochemical response of infected plants were evident with the activities and formation of oxidase,peroxidase,polyphenol,cellulose and lignin.The oxidase were more active in resistant cultivar than that in the healthy ones.Colour comparison test combined with Ultra-Violet absorption method indicated that the accumulated concentration of polyphenol in the infected tissues was as high as 100 ppm and the contents of cellulose and lignin in dise- ased branch were significantly lower than that in healthy ones,resultingiin“soft shoot”symptom.The ac- tivity of spiroplasma was inhibited by soaking the diseased parts of host plants in50-55℃ water and 500 ppm oxytetracycline as well as inactivated by the treatment of microwave.
出处 《浙江农业学报》 CSCD 1990年第3期127-131,共5页 Acta Agriculturae Zhejiangensis
关键词 皱褶螺原体 生化反应 理化抗性 Mulberry Spiroplasma Biochemical reponse Physicochemical resistivity
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