摘要
初步研究了盐分胁迫对水稻幼苗和愈伤组织生长的影响,并探讨了外源脯氨酸的作用。结果表明,水稻幼苗和愈伤组织的生长,随氯化钠浓度升高均而明显降低。在盐分胁迫下幼苗中积累游离脯氨酸,并随盐浓度升高而增加。籼稻品种脯氨酸积累较粳稻品种多。脯氨酸积累量与幼苗受盐害程度呈相反趋势。过氧化物酶活性在盐浓度为1.5%时最强。该酶活性变化与幼苗受害程度未见明显联系。过氧化物酶和细胞色素氧化酶分布在幼苗主根横切面上。不同处理间无明显差异。外源脯氨酸能明显减轻盐分对愈伤组织生长的抑制,但对幼苗生长影响不明显。在无盐分胁迫下脯氨酸明显促进水稻愈伤组织的生长。
The present study was designed to examine the effec of salt stress on the growh of seedling and callus of rice.The rule of exogenous proline was also studied.Results showed that with the increase of NaCl concentrations the growth of seedling and callus of rice was significantly decreased and free proline accumulated in rice seedlings under salt stress.The proline accumulation was found much more in indica than in japonica rice.The injury of seedlings which accumulated more free proline was less severe under salt stress.The activity of peroxidase reached its top level as NaCl concentration increased to 1.5%.The variation of peroxidase activity was much larger in japonica than in indica.There was no significant rela- tion between peroxidase activity and salt injury of seedlings.Peroxidase and cytochrome oxidase distrib- uted in transversal section of main roots.Not many differences were observed between treatments. Exogenous proline could reduce the inhibitor from salt on the growth of callus,but not on seedlings,Un- der free salt stress,proline promoted the growth of callus.
出处
《浙江农业学报》
CSCD
1990年第3期106-110,共5页
Acta Agriculturae Zhejiangensis
