摘要
目的比较薄荷全组分、水提组分、挥发油对小鼠的急性毒性作用。方法分别制备薄荷全组分、水提组分和挥发油,按照经典小鼠急性毒性试验方法,进行薄荷不同组分的小鼠急性毒性试验比较研究,试验数据用公斤体重法计算半数致死量(LD50)、最大耐受量(MTD)和最大给药量(MLD),连续观察14 d,记录小鼠毒副反应情况。结果测得薄荷挥发油LD50为5.9777%相当于(1.4895 mL.kg-1.d-1)9,5%的可信限为(5.4291～6.5807)%相当于(1.3508～1.6407)mL.kg-1.d-1;水提组分MTD值按含生药量计算分别为64.0 g.kg-1.d-1,相当于临床70 kg人每公斤体重日用量的746.7倍;全组分MLD值按含生药量计算分别为24.36 g.kg-1.d-1,相当于临床70 kg人每公斤体重日用量的284.2倍。结论薄荷不同组分对小鼠急性毒性强度为:薄荷挥发油>薄荷水提组分>薄荷全组分,其毒性成分与中毒机理尚待进一步研究。通过对薄荷不同组分急性毒性的比较,为薄荷的临床安全用药提供实验依据。
Objective To compare the acute toxicity of different components in Herba Menthae on mice which is all components, peppermint oil, water extract, make an observation and evaluation of acute toxicity to Herba Mentbae in order to provide a basis for safe and reasonable medication in clinical. Methods Respectively prepare the all components, peppermint oil, water extract, and compare the acute toxicity of different components in Herba Menthae on mice in accordance with classical acute toxicity test methods, and the experimental datas are calculated LD~, with the Bliss method, with the continuous administration and observation for 14 days. Results The LD50 of peppermint oil is 1.4895 ml·kg^-1·d^-1 and the 95%confidence interval is 1.3508-1.6407 mL·kg^-1·d^-1; the water extract in Herba Menthae are unable to make LD50 MTD results calculated in accordance with crude drug content were respectively 64.0g·kg ^-1·d^- 1, which is equal to 746.7 times of 70kg people＇s daily dried medicinal herb expenses, the MLD of all component is 24.36 g·kg^-1·d^-1＇, which is equal to 284.2 times of 70kg people＇s daily dried medicinal herb expenses. Corlclusion The acute toxicity of different components in Herba Menthae on mice is： peppermint oil〉 water extract〉 all components. By comparing the acute toxicity of different components in Herba Menthae on mice, it is beneficial to make an observation and evaluation of acute toxicity to Herba Menthae herbs, provide experimental basis for further toxicity-based parts and toxical substances and provide a basis for safe and reasonable medication in clinical.
出处
《中国药物警戒》
2012年第2期65-68,共4页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题(2008GG2NS02021)
关键词
薄荷
不同组分
急性毒性
比较
Herba Menthae
different components
mice
acute toxicity
compare
