摘要
目的探讨柴胡总皂苷醇洗脱精制品对小鼠急性毒性的影响。方法采用经典的小鼠急性毒性试验方法,进行柴胡总皂苷醇洗脱精制品对小鼠的急性毒性研究。结果按含柴胡总皂苷含量计算,柴胡总皂苷醇洗脱精制品对小鼠的半数致死量(LD50)为446.7mg·kg-1·d-1,95%可信限为386.13～513.66mg·kg-1·d-1。死亡小鼠急性毒性主要表现为安静怠动,即而出现走路不稳、腹卧昏睡、心率加快、呼吸急促、连续性抽搐、神经抑制而死亡。存活小鼠在14天观察期内体重增长缓慢,余未见明显异常。结论柴胡总皂苷醇洗脱精制样品的小鼠急性毒性比柴胡总皂苷粗提取物的急性毒性大,进一步证实了柴胡总皂苷是柴胡产生毒性的主要物质基础。其体内蓄积毒性的"量-时-毒"关系有待进一步研究。
Objective To discuss the effect of refined products of Bupleurum saikosaponin by alcohol elution on mice＇s acute toxicity.Methods Make the study on mice＇s acute toxicity of the refined products of Bupleurum saikosaponin by alcohol elution in accordance with classical acute toxicity test methods.Results Caculated by total saikosaponin,the LD50 of the refined products of Bupleurum saikosaponin by alcohol elution is 446.7 mg·kg-1·d-1,and the 95% confidence interval is 386.13～513.66 mg·kg-1·d-1.The main acute toxicity symptoms and signs of dead mice are negligent action,then arising from unsteady gait,abdominal lying sleeping,rapid heart rate,shortness of breath,continuous convulsions,neurological inhibition,and eventually to death.The surviving mice＇s weight gainings are slow in 14d obervation period and there are noother obvious abnormalities.Conclusion The acute toxicity of refined products of Bupleurum saikosaponin by alcohol elution is bigger than the total Bupleurum saikosaponin extracts,and we further confirm that the total saikosaponin is mainly toxical material basis of Bupleurum.The ＂dose-time-poison＂ relationships of accumulated toxicity are to be further studied.
出处
《中国药物警戒》
2010年第9期527-529,共3页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金项目(30672649)
山东省科技平台建设项目(2008GG2NS02021)
山东省科技公关关键技术研究课题(2007GG2NS02073)
山东省国际合作引智项目(L20083700336)
国家863科技攻关计划课题(2006GG2202065)
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
关键词
柴胡总皂苷
分离精制
小鼠
急性毒性
total saikosaponin
separation and purification
mice
acute toxicity
