摘要
[目的]为科学利用老瓜头及其内生真菌提供参考。[方法]采用多种色谱分离技术,对老瓜头内生真菌LGT-L4发酵物乙酸乙酯萃取部分进行分离纯化,并通过核磁共振(NMR)、质谱等波谱方法对分离得到的化合物进行结构鉴定。[结果]从老瓜头内生真菌LGT-L4发酵物乙酸乙酯萃取部分分离得到5个化合物,分别为对羟基苯甲醛、尿嘧啶苷、L-焦谷氨酸、甲基焦谷氨酸和蔗糖。[结论]明确了老瓜头内生真菌LGT-L4代谢产物。
[Objective] The aim was to provide references for using C.komarovii and its endophytic fungus scientifically.[Methods] Constituents were isolated from ethyl acetate portion of endophytic fungus LGT-L4 leavening from C.komarovii by modern chromatographic techniques and their structures were determined by physic-chemical evidences and spectroscopic methods such as NMR and mass spectra.[Results] Five known chemical constituents were isolated from ethyl acetate portion of endophytic fungus LGT-L4 leavening from C.komarovii,identified as parahydroxybenzaldehyde,uridine,L-focal glutamic acid,focal glutamic acid methyl and sucrose.[Conclusion] The metabolites of endophytic fungus LGT-L4 from C.komarovii were defined.
出处
《安徽农业科学》
CAS
2012年第3期1417-1418,共2页
Journal of Anhui Agricultural Sciences
基金
国家自然基金项目(30960465)
宁夏自治区科技攻关项目(回药老瓜头内生真菌活性筛选及发酵工艺研究)
关键词
老瓜头
内生真菌
化学成分
发酵
Cynanchum komarovii Al.Iljinski
Endophytic fungus
Chemical constituents
Fermentation
