摘要
目的:观察牵张成骨区早期快速移动牙压力侧牙周组织的改建,为牵张成骨区早期快速牙移动的安全性和可行性提供实验依据。方法:选择10只雄性Beagle犬,采用自身对照设计,随机选择犬的一侧下颌骨行牵张成骨术作为实验组,利用正畸种植钉作为支抗,牵张完成后,即刻以30g力远中移动第3前磨牙(P3)进入牵张成骨区。对侧拔除下颌第四前磨牙(P4)后,以30g力同期远中移动P3作为对照组。每周用游标卡尺测量第三前磨牙远中移动的距离,每个距离测量3次,取其平均值并记录,采用SPSS18.0软件包对相关数据进行配对t检验。分别于牙移动1、2、4周后,取材行HE染色及抗酒石酸酸性磷酸酶(TRAP)染色,观察移动牙的压力侧牙周组织的变化。结果:牵张成骨区牙平均移动速度达到每周(1.055±0.054)mm,明显快于对照组,差异具有统计学意义(P<0.05),且无明显迟滞阶段。实验组移动牙的牙周组织压力侧未观察到透明样变性,牙根表面吸收陷窝少见,而TRAP染色阳性细胞的数量和面积均大于对照侧,且表达更强。结论:牵张成骨新骨区牙移动速度明显加快,无明显迟滞阶段;可能与移动牙牙周组织的压力侧未观察到透明样变性,破骨细胞出现早,范围广,破骨活动更为活跃有关。
PURPOSE:To investigate periodontal remodeling mechanism on the compression side during early tooth movement into newly distracted bone.METHODS:Ten male Beagle dogs were selected.Distraction osteogenesis was performed on randomized side as experimental group,while the fourth premolars were extracted on the other side as control group.Then the third premolars were distalized with 30g orthodontic force instantly after the cessation of distraction or extraction.The distance of the tooth movement was measured with a sliding caliper every week.Each distance was measured 3 times and the mean value was recorded.The measurement data were analysed with paired t test by SPSS 18.0 software package.Beagle dogs were killed in the first,second,fourth week after tooth movement.Slices were obtained for HE staining and TRAP staining to observe the periodontal tissue on the compression side.RESULTS:The average moving velocity of the teeth in the distracted bone was(1.055±0.054)mm per week,which was significantly faster than that in the control group(P0.05).There was no apparent lag period in the experimental group.In addition,there was no hyalinization observed on the compression side of the periodontal tissue in the experimental group,while the amount and area of distribution of the TRAP-positive cells on the compression side was significantly larger and strongly expressed.CONCLUSIONS:The moving velocity of the teeth in the newly distracted bone was significantly faster,and no apparent lag period,which may be related to no hyalinization and more early-appeared,vigorous and wide-distributed osteoclasts on the compression side of the periodontal tissue.Supported by Research Fund of Science and Technology Commission of Shanghai Municipality(08DZ2271100).
出处
《上海口腔医学》
CAS
CSCD
2011年第6期584-589,共6页
Shanghai Journal of Stomatology
基金
上海市科学技术委员会资助项目(08DZ2271100)
关键词
牵张成骨
牙移动
破骨细胞
牙周组织
Distraction osteogenesis
Tooth movement
Osteoclast
Periodontal tissue
