摘要
目的了解内蒙古呼和浩特市2008年6月-2010年5月致婴幼儿腹泻A组轮状病毒的流行特征及病毒基因亚型特点。方法收集内蒙古妇幼保健医院<5岁全部住院腹泻患儿粪便标本730份,进行轮状病毒抗原检测,对检测阳性标本用巢式反转录聚合酶链式反应(RT-PCR)法进行分型鉴定。结果 730份标本中,酶联免疫法(ELISA法)检出阳性标本298份,阳性率为40.8%,对298份轮状病毒抗原阳性标本进行RT-PCR检测,其中G血清型分出亚型的有292份,占98.0%,P基因型分出亚型的有254份,占85.2%;同一份标本G、P分型方法均分出亚型的有250份,占83.9%,同时分出98份G亚型组合型,11份P亚型组合型。结论 G1,G3,P〔8〕是流行的优势亚型,G1+G3组合亚型又是主要的组合流行型;呼和浩特市轮状病毒感染高发季节是10月至次年2月,流行优势毒株发生了从G1到G3的漂移。
Objective To investigate epidemic characteristics and genotypes of group A human rotavirus in infants and young children with diarrhea in Hob_hot from June 2008 to May 2010. Methods Totally 730 stool specimens were collected from children under 5 years old and with diarrhea. Enzyme-linked immunosorbent assay（ELISA）was used to detect rotavirus antigen. The ELISA positive samples were detected with nest reverse transcription-polymerase chain reaction （RT-PCR） to determine the G and P genotypes of rotavirus. Results Among the samples ,298 were rotavirus antigen positive （40.8 % ） ; 292 were G genotype positive（98. 0% ） ;254 were P genotype positive（85.2% ） ;and 250 samples had G and P genotypes （83.9%）. Couclusiou G1, G3 and P[ 8 ] were the predominant genotypes and G1 ＋ G3 was the predominant mixture subtype for the epidemic of group A rotavirus in Hohhot and there was a drift of predominant genotype from G1 to G3 from 2008 to 2010.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2012年第1期81-82,共2页
Chinese Journal of Public Health
基金
内蒙古自治区卫生厅医疗卫生科研项目(2010111)
关键词
A组轮状病毒
腹泻
血清亚型
基因亚型
组合型
group A human rotavirus
diarrhea
sub-serotype
sub-genotype
recombination type
