摘要
目的:了解辽宁省腹泻患儿中人轮状病毒(Human Rotavirus,HRV)和人星状病毒(Human Astrovirus,HAstV)感染情况。方法:采集5岁以下腹泻患儿粪便标本131份,用聚丙烯酰胺凝胶电泳(PAGE)检测人轮状病毒,阳性标本用反转录-聚合酶链反应(RT-PCR)进行血清G分型和基因P分型。用RT-PCR检测人星状病毒,阳性标本进行测序、碱基序列比对,并构建开放读码框ORF2进化树。结果:131份粪便标本中,人轮状病毒PAGE检测阳性29份(22.1%),其中G血清型:G3型13份(44.8%),G1+G3型2份(6.9%),未分型14份(48.3%);P基因型:P[8]型18份(62.1%),P[4]型1份(3.4%),未分型10份(34.5%)。人星状病毒RT-PCR检测阳性2份。通过对这2株人星状病毒ORF2基因核苷酸碱基序列进行比对及进化树分析,发现它们与在日本流行的基因1型人星状病毒参考株高度同源。结论:人轮状病毒是引起我省散发婴幼儿腹泻的主要病原;检测到的2株人星状病毒属于1型人星状病毒,这是我省首次检测出人星状病毒。
Objective：To investigate human rotavirus and human astrovirus infection of children in Liaoning Province.Methods：131 stoo1 specimens were collected from children who were younger than 5 years old with diarrhea and were used for the detection of human rotavirus by polyacrylamide gel electrophoresis（PAGE）.Serotyping G and genotyping P identifications of human rotavirus were determined by reverse transcription polymerase chain reaction（RT-PCR）.Human Astrovirus was detected by RT-PCR and positive samples were sequenced and compared,and phylogenetic tree of open reading frame 2（ORF2） was constructed.Results：Of the total 131 stool specimens,29（22.1%） Human Rotavirus strains were detected by PAGE.Of 29 positive samples,G serotyping as G3 was 13（44.8%）,G1＋G3 2（6.9%）and nontypeable 14（48.3%）;P genotyping,in turn,P[8]accounted for 18（62.1%）,P[4]1（3.4%）,and nontypeable 10（34.5%）.2 Human Astrovirus strains were detected by RT-PCR and sequenced for their ORF2,and nucleotide sequence comparison and phylogenetic analysis showed 2 strains were the highest identical to those genotype 1 strains which prevalent in Japan.Conclusion： Human Rotavirus is the most important viral agent sporadic causing diarrhea among children under 5 years old in Liaoning Province.Two human astrovirus strains belong to genotype 1 which were detected in Liaoning Province for the first time.
出处
《中国卫生检验杂志》
CAS
2009年第3期643-645,共3页
Chinese Journal of Health Laboratory Technology
基金
辽宁省博士科研基金项目(20052135)
