摘要
目的:建立一种反相高效液相色谱法快速测定油茶籽油中苯并(a)芘含量的新方法。方法:样品经溶剂提取后直接进行检测,采用岛津InertsilODS-SPC18色谱柱,以90%乙腈-10%水为流动相,流速为1.0mL/min,荧光检测器。结果:苯并(a)芘的浓度在0.5～50μg/mL范围内时,峰面积与含量具有良好的线性关系(r=0.9994);方法的精密度良好,平均相对标准偏差为1.01%,平均加标回收率为93.8%。结论:前处理简单、结果准确可靠、方法易于推广。
Objective： A method for rapid detection of benzo （a） pyrene in the oil-tea camellia seed oil was established by means of RP-HPLC.Methods：The sample after being treated with solvent was directly determined under the following conditions： Shimadzu inertsil ODS- SP column, acetonitrile-water （90/10） at the flow speed of 1.0mL/min, and the fluorescence detector. Results： A good linear relationship of peak area with benzo （a） pyrene content occurred（ r = 0.9994）when the concentration of benzo （a） pyrene was from 0.5-50pg/mL. The average relative standard deviation was 1.01% and the average recovery of added standard sample was 93.8%. Conclusion ：The pretreament was simple,rapid,accurate and easy to popularize.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第2期64-66,共3页
Science and Technology of Food Industry
基金
湖南省科技计划项目(2010WK2004
2010CK3024)
湖南省研究生科研创新项目(CX2009B184)
关键词
高效液相色谱
荧光检测器
苯并(A)芘
油茶籽油
high performance liquid chromatography
fluorescence detector
benze （a） pyrene
oiltea camellia seed oil
