摘要
目的:建立清肺合剂的高效液相色谱指纹图谱。方法:色谱柱为ElitSinoChromODS-AP(250mm×4.6mm,5μm),柱温为50℃,流动相为0.01%磷酸溶液-乙腈(梯度洗脱),流速为1.0mL.min-1,检测波长为330nm。结果:建立了16批清肺合剂的对照指纹图谱,得到共有峰16个,其中对香豆酸峰为参照峰。各批次样品的指纹图谱及对照图谱间相似度高。结论:所建方法准确、简便、稳定性好,可为清肺合剂的生产和质量控制提供依据。
OBJECTIVE： To establish a HPLC fingerprint of Qingfei mixture. METHODS ： The optimal condition of separation and detection were achieved on an Elite SinoChrom ODS-AP （250 mm×4.6 mm, 5 μm） Column with mobile phase consisted of acetonitrile and 0.01% phosphoric acid aqueous solution （gradient elution） at flow rate of 1.0 mL.min^-1.The detection wavelength was set at 330 nm.RESULTS： Control fingerprints of 16 batches of Qingfei mixture were established and 16 peaks were regarded as the characteristic peaks. The peak of p-coumaric acid was appointed as the reference peak. According to the similarity, the diversity of quality among different samples was minor. CONCLUSION： Established method is accurate, simple and stable. It provides basis for the production and quality control of Qingfei mixture.
出处
《中国药房》
CAS
CSCD
2012年第3期256-258,共3页
China Pharmacy
基金
浙江省卫生高层次创新人才培养工程项目
浙江省中医药科技计划项目(2009JA001)
