摘要
为了降低辣椒采后由灰霉病引起的腐烂损失,从辣椒植株内分离筛选拮抗菌,结果获得6株对辣椒灰霉病菌有较强抑制作用的内生细菌,其中PL58菌株的抑菌活性最强,在平板上培养3d后抑菌圈直径大于39 mm,培养7 d后对辣椒灰霉病菌的抑制率达55.1%,该菌株初步鉴定为芽孢杆菌,其拮抗作用有一定的广谱性。该菌株的发酵液(1.0×109 cfu/mL)对辣椒灰霉病菌菌丝生长和孢子萌发均有较强的抑制作用,但其无菌滤液没有抑制作用。该菌株在辣椒果实上对灰霉病的拮抗效果最高达78%,辣椒果实在25℃下贮藏25 d后该菌株的生防效果仍有37.2%。因此,PL58菌株可以作为一种生防菌应用于辣椒采后灰霉病的防治。
For the purpose of reducing pepper postharvest rot caused by gray mold rot, this study was carried out to isolate and screen antagonistic bacteria from pepper plants. The result showed that 6 strains of antagonist which could effectively control the postharvest gray mold rot of pepper fruits were isolated, among which the strain PL58 showed the most effective antagonizing activity, and its inhibition circle diameter was more than 39 mm after incubated on the medium plate for 3 d, while the inhibition rate to the pathogeny of pepper gray mold rot was 55.1% after incubated for 7 d, and the strain PL58 which had the characteristic of wide-spectrum antagonism was identified as Bacillus sp. preliminarily. The suspension（1.0×10^9 cfu/mL）of the strain showed strong inhibiting effect to mycelial growth and spore germination of the pathogeny, however, its sterile cellular exudates showed no significant inhibiting effect. Further studies showed that the strain had the best antagonizing effect to the gray mold rot up to 78% on pepper fruits and the best bio-control effect was 37.2% after the fruits stored at 25℃ for 25 d. As a result, the strain PL58 could be applied as a bio-control agent to control pepper postharvest gray mold rot.
出处
《热带作物学报》
CSCD
2011年第11期2118-2122,共5页
Chinese Journal of Tropical Crops
基金
中央级公益性科研院所基本科研业务费专项资金资助项目(No.sscri201004
No.sscriqd200808)
海南省自然科学基金(No.809040)
关键词
辣椒
灰霉病
拮抗细菌
生防效果
Pepper
Gray mold rot
Antagonistic bacteria
Biocontrol efficacy
