摘要
目的建立高效液相色谱(HPLC)法同时测定清热解毒口服液中绿原酸和黄芩苷含量的分析方法。方法用C_(18)硅胶柱(150 mm×4.6mm,5 gm)为固定相,甲醇-0.4%的磷酸水溶液为流动相,进行梯度洗脱,流速为1 mL/min,紫外检测波长为327 nm。结果绿原酸和黄芩苷的线性范围分别为3.97-99.2μg/mL和16.24-406.0μg/mL;相关系数分别为0.999 4和0.999 5;加样回收率分别为99.92%和100.31%;检出限分别为0.07μg/mL和0.12μg/mL;精密度实验RSD分别为1.08%和1.21%;重现性实验RSD分别为0.23%和0.78%;稳定性实验RSD分别为1.99%和1.09%;3批样品中绿原酸的含量在0.126-0.238 mg/mL,黄芩苷的含量在1.157-3.079 mg/mL。结论该方法简便、快速、准确、灵敏度高、重现性好,可用于清热解毒口服液的质量控制。
Objective To develop an RP - HPLC method for the determination of chloregenie acid and baiealin in Qingrejiedu oral liquid. Methods The chromatographic column was Kromasil C18 （ 150 × 4.6 mm, 5 um）, The mobile phase was CH3 OH -0.4% Phosphoric acid. The flow rate was set at 1.0ml/min. The detection wavelength was at 327 nm. Chlorogenic acid and baiealin were separated by HPLC with grade elution. Results The linearity ranges of chlorogenie acid and baicalin were 3.97 - 99.2 ug/mL and 16.24 - 406.0 ug/mL respectively, the correlations coefficients were 0.999 4 and 0.999 5, the average recoveries of adding sample were 99.92 % and 100.31%, the detection limits were 0.07 ug/mL and 0.12 ug/mL, the RSD （ n = 5 ） of measurement precisions test were 1.08% and 1.21% , the RSD （n = 5） of reproducibility between tests were 0.23% and 0.78%, the RSD （n =5） of stability test were 1. 99% and 1. 09%. The content of chlorogenic acid in compound Qingrejiedu oral liquid was 0. 126 - 0.238 mg/mL, and that of baicalin was 1. 157 -3. 079 mg/mL. Conclusion The method is simple, fast, accurate, sensitive and reproducible. It is fit for the quality control of compound Qingrejiedu oral liquid.
出处
《湖北中医药大学学报》
2011年第6期33-35,共3页
Journal of Hubei University of Chinese Medicine
