摘要
为了获取优质高产的蛹虫草,菌丝体生长速度和生长是否健壮是最主要的影响因素。通过单因素试验找出了菌丝体的最适生长指标,采用均匀试验优化培养基中各种成分添加量。研究得出,乳糖和蛋白胨为菌丝体培养的最佳碳氮源。优化后的培养基组分为：蛋白胨2.5g/L,乳糖39.9g/L,KH2PO40.5g/L、MgSO4·7H2O3.2g/L和CaCl20.3g/L;适宜温度是15~28℃,最适为25℃;适宜pH范围为5~9,最适为7.5;无机盐对菌丝体生长速度影响不大,但对菌丝生长势有一定的影响,光照会抑制菌丝体的生长,以暗培养为宜。在此条件下通过试验证明,菌丝体长速可达到4.0mm/天,且菌落菌丝呈现出浓密、洁白、粗壮有光泽。
In order to obtain the high yield and quality of Cordyceps militaris,it was the main influencing factors that affected growth rate and strong of mycelia.The author found out the optimal growth index of mycelia by single factor experiment,and optimization various additions of medium components by the uniform test.Research showed that lactose and peptone were the best carbon and nitrogen source for the cultivation of mycelia.Optimization of medium components of mycelium obtained：peptone was 2.5 g/L,lactose was 39.9 g/L,KH2PO4 was 0.5 g/L,MgSO4.7H2O was 3.2 g/L and CaCl2 was 0.3 g/L;the suitable temperature for the mycelium of this fungi growth was ranged from 15-28℃with the optimum temperature being 25℃,the suitable pH was ranged from 5-9 with the optimum pH being 7.5,inorganic salts had little effect on the mycelium growth rate,but it was to a certain extent mycelial growth potential,light could inhibit the growth of mycelium,the dark culture was appropriate.In these conditions through the experiments demonstrated that the growth speed of mycelia could reach 4.0 mm/d,and Cordyceps colony presented a thick,white,hairchested and luster.
出处
《中国农学通报》
CSCD
北大核心
2011年第31期120-125,共6页
Chinese Agricultural Science Bulletin
基金
"十一五"国家科技支撑计划(2007BAE42B04)
关键词
蛹虫草
菌丝体
固体培养
优化条件
Cordyceps militaris（Fr.）Link
mycelium
solid culture
optimum conditions
