摘要
目的:观察并分析桂枝芍药知母汤对大鼠急性痛风性关节炎血清抗炎因子白介素1(IL-1)、促炎因子白介素4(IL-4)含量的影响,探讨该方治疗急性痛风性关节炎的作用机制。方法:将雄性Wister大鼠40只,随机分为4组,即空白对照组(A组)、模型对照组(B组)、秋水仙碱对照组(C组)、桂枝芍药知母汤组(D组),按国外Coderre等经典方法,在受试大鼠右侧踝关节腔内注入尿酸钠溶液,形成痛风模型;A组以同样方法注射0.2 mL生理盐水;C组、D组分别在造模前灌胃秋水仙碱、桂枝芍药知母汤,A组、B组分别灌胃等剂量生理盐水。于造模后72 h断尾采血,抽取关节液,观察各组血清IL-1、IL-4含量及关节液中WBC的变化。结果:桂枝芍药知母汤组大鼠血清中IL-1含量明显低于模型对照组(P<0.01)、秋水仙碱组(P<0.05),血清中IL-4的含量明显高于模型对照组(P<0.01)、秋水仙碱组(P<0.05),且关节液中WBC渗出明显低于模型对照组(P<0.05),但与秋水仙碱组比较差异无统计学意义(P>0.05)。结论:桂枝芍药知母汤可能是通过抑制IL-1及促进IL-4的表达来达到抑制急性痛风性关节炎炎症的目的。
Objective： To observe and analyze Guizhishaoyaozhimu Tang on acute gouty arthritis serum anti-inflammatory factor interleukin 1 （IL-1）, pro-inflammatory cytokines interleukin 4 （IL-4） content of effects of the treatment of acute gouty arthritis side mechanism of action. Methods： 40 male Wister rats were randomly divided into four groups, namely, the control group （A）, model control group （B）, colchicine control group （C group）, group Guizhishaoyaozhimu soup （D）, according to foreign Coderre and other classical methods, the subjects right ankle articular injection of rat sodium solution, the formation of gout model; A group injected with 0.2 mL of saline in the same way; C group, D group at before modeling fed colchicine, Guizhishaoyaozhimu soup, together with the contrast in order to form, A group, B group were fed doses of saline, etc. 72 h after modeling tail blood, synovial fluid extraction, observed the serum IL-1, IL-4 levels and synovial fluid WBC changes. Results： Guizhishaoyaozhimu soup of serum IL-1 were significantly lower than the control group （P〈0.01）, colchicine group （P 〈0.05）, serum levels of IL-4 was significantly higher than the model the control group （P〈0.01）, colchicine group （P〈0.05）, and synovial fluid WBC leakage was significantly lower than the control group （P〈0.05）, but with the colchicine group difference was not statistically significant （P〉0.05）. Conclusion： Guizhishaoyaozhimu soup may be through inhibition of IL-1 and promote the expression of IL-4 to suppress inflammation in acute gouty arthritis purpose.
出处
《中医药导报》
2011年第12期16-18,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省教育厅基金资助(09C723)
湖南中医药大学中医临床基础重点学科资助
