摘要
目的:研究影响美洲大蠊药材中游离氨基酸含量测定的各因素,确定最佳检测条件。方法:采用单因素实验和正交设计实验,对溶剂浓度、物料比、提取时间、提取次数、活性炭用量、脱色时间、缓冲溶液的pH值、显色时间进行考察。结果:美洲大蠊药材中游离氨基酸的最佳提取条件为:8倍量65%乙醇,回流提取3次,每次60 min;最佳测定条件为:脱色时活性炭和样品的比例为4∶100,脱色时间10 min,显色时缓冲溶液的pH=6.0,水浴加热显色30 min。结论:该提取条件和测量方法适用于美洲大蠊中游离氨基酸的含量测定。
Objective： To study the influence factors of the determination of free amino acids in Periplaneta americana medicine and find out the best testing condition.Methods： Single factor experiment and orthogonal test design were applied to determine the solvent concentration,material ratio,extraction time,extraction times,active carbon dosage,decoloring time,pH value of the buffer solvent and coloring time.Results： The optimum extraction condition for free amino acids from the Periplaneta americana is that reflux extraction is applied to the 8 times of 65% ethanol for 3 times with each time lasting 60 minutes;the optimum testing condition lies when the ratio between active carbon and the sample is 4：100 in the 10 minutes＇ decolorization,and the pH value of the butter solvent is 6.0 in coloring and then water bath heating is applied for 30 minutes.Conclusion： The extraction and testing conditions are suitable for testing the amount of free amino acids in Periplaneta americana.
出处
《大理学院学报（综合版）》
CAS
2011年第12期12-15,共4页
Journal of Dali University
基金
国家自然科学基金项目(81060329)
关键词
美洲大蠊
游离氨基酸
含量测定
影响因素
Periplaneta americana
free amino acids
content determination
influence factor
