摘要
目的:探讨急慢性乙醇中毒对大鼠心脏的影响机制.方法:雄性健康SD大鼠,随机分为急性和慢性对照组、急性和慢性乙醇中毒组,每组8只.酶法和比色法检测血清和心肌组织谷草转氨酶(AST)、肌酸磷酸激酶(CPK)、超氧化物歧化酶(SOD)、丙二醛(MDA)、Ca2+-Mg2+-ATPase和Na+-K+-ATPase的含量,观察心肌微细结构的改变.结果:与对照组相比:急、慢性乙醇中毒组心肌MDA、AST、CPK和血清AST、CPK含量升高(P<0.05,P<0.01),SOD含量减少(P<0.05,P<0.01),Na+-K+-ATPase和Ca2+-Mg2+-ATPase活性降低(P<0.05,P<0.01).心肌组织HE染色显示,乙醇中毒组大鼠心肌细胞肿胀、细胞界限模糊不清,慢性组尤为明显.结论:急慢性乙醇中毒通过对大鼠心肌酶和氧自由基的影响而对心脏产生抑制作用.
Objective： To investigate the intervention mechanism of acute and chronic alcoholism on rats＇ heart. Methods： Healthy male SD rats were randomly divided into the control group and alcoholism group which were treated with acute and chronic group respectively,8 rats of each group. To detect the aspartate aminotransferase （AST）, creatine phosphokinase （ CPK ）, superoxide dismutase （SOD）, malondialdehyde （MDA）, Ca^2＋ -- Mg^2＋ -- ATPase and Na^＋ -- K^＋ -- ATPase, enzymic method and colorimetric method were both employed. To observe the fine structure of myocardium by HE staining. Results compared with control group, the contents of MDA, CPK, AST increased （P〈0.05, P〈0.01 ）, while those of SOD, Na^＋ -- K^＋ --ATPase and Ca^2＋ --Mg^2＋ --ATPase reduced（P〈0.05,P〈0.01）. HE staining of myoeardium showed that the myocardium cell swelled and the cell outline confused in alcoholism group, especially the chronic alcoholism group. Conclusion acute and chronic alcoholism had inhibition on rats＇ heart by the inflluence of myocardium enzymes and oxygen free radicals.
出处
《德州学院学报》
2011年第6期61-64,共4页
Journal of Dezhou University
基金
山东省自然科学基金(ZR2010CL018)
山东省德州市科技计划基金(20090162-4)
山东省卫生厅资助项目(2009HZ005)
关键词
急慢性乙醇中毒
心肌酶
氧自由基
acute and chronic alcoholism
myocardium enzyme
oxygen free radical
