摘要
目的 观察免疫吸附特异性清除循环肿瘤坏死因子-α(TNF-α)对内毒素休克时肝细胞氧化应激的影响,并探讨其与一氧化氮(NO)之间的关系。方法 给新西兰白兔一次性静注内毒素(LPS)80×109cfu/kg,免疫吸附组和空灌流组分别于1小时后经抗TNF-α单克隆抗体亲和免疫吸附柱或未偶联抗体的空灌流柱进行血液灌流2小时,对照组不予血液灌流,观察血浆NO-2和丙二醛(MDA)含量、全血超氧化物歧化酶(SOD)活力、肝组织中MDA含量和SOD活力以及肝功能变化。结果 免疫吸附治疗后,血浆和肝组织中MDA含量明显下降,全血和肝组织中SOD活力显著增高;血浆NO的生成显著减少,但又高于注射LPS前水平;肝功能损害明显减轻。结论 免疫吸附特异性清除循环TNF-α既明显抑制了内毒素休克时肝细胞氧化应激,又使NO浓度保持在一定范围内,可能是该方法能明显减轻内毒素性肝损害的重要原因。
Objective To evaluate the effect of specific removal of circulating tumor necrosis facfor-α(TNF-α) by immunoadsorption on hepatocellular oxidant stress during endotoxin shock.Methods New Zealand white rabbits were injected intravenously with Lipopolysaccharide(LPS,Escherichia coli O 111 :B4,8 0×10 9 cfu/kg)and undergone hermoperfusion through a column containing immunoadsorbent produced by the attachment of anti-TNF-α monoclonal antibody to agarose beads at 1st hour after LPS injection.The plasma nitrite (NO - 2,one of the stable end products of nitric oxide)and malondialdehyde(MDA)levels,blood superoxide dismutase(SOD)activities,as well as serum alanine transaminase and aspartate transaminase levels were detected,At 24th hour after injecting LPS,the MDA levels and SOD activities of livers were measured also.Results After immunoadsorption treatment, the concentration of plasma NO 2 - was significantly decreased but it was significantly higher than the baseline value,and reduced productions of oxygen free radicals,as well as attenuated injury of liver function were observed.Conclusion Specific removal of circulating TNF-α by immunoadsorption can reduce the production of oxygen free radicals and inhibit hepatocellular oxidant stress. [
出处
《中国急救医学》
CAS
CSCD
北大核心
2000年第1期1-3,共3页
Chinese Journal of Critical Care Medicine
基金
国家自然科学基金!批准号 :3 93 70 3 3 6
关键词
内毒素休克
肿瘤坏死因子Α
肝脏
氧自由基
Shock,septic
Tumor necrosis factor
Immunoadsorption
Liver
Oxygen free radicals
