摘要
目的探讨西维因与氰戊菊酯联合染毒对雄性大鼠睾丸的脂质过氧化作用以及对精子参数和血清激素水平的影响。方法选择健康成年清洁级雄性SD大鼠40只,按2×2析因设计随机分为4组,分别为阴性对照(玉米油)组、西维因单独染毒组(1/50 LD50,11.2 mg/kg)、氰戊菊酯单独染毒组(1/50 LD50,9.02 mg/kg)和西维因(11.2 mg/kg)+氰戊菊酯(9.02mg/kg)染毒组,每组10只。采用经口灌胃方式进行染毒,每天1次,每周6 d,连续染毒8周。末次染毒24 h后,测量大鼠体重,睾丸和附睾脏器湿重(并计算脏器系数),精子参数(精子计数、精子存活率、精子畸形率),睾丸组织中超氧化物歧化酶(SOD)、谷胱甘肽S转移酶(GST)活力和还原性谷胱甘肽(GSH)、丙二醛(MDA)含量,血清中雄性激素睾酮(T)、黄体生成素(LH)、促卵泡激素(FSH)水平。结果西维因和氰戊菊酯联合染毒对大鼠体重、睾丸和附睾的脏器系数均无交互作用(P>0.05);对精子存活率和畸形率均存在拮抗作用,对精子计数无交互作用(P>0.05);对睾丸组织中SOD活力、MDA含量均存在拮抗作用(P<0.01,P<0.05),对GST活力和GSH含量均无交互作用(P>0.05);对血清中T、FSH、LH均存在拮抗作用(P<0.05,P<0.01)。结论西维因和氰戊菊酯联合染毒对雄性大鼠生殖系统存在联合毒性作用,可能是通过破坏氧化-抗氧化系统的平衡和影响睾丸组织中酶的活力、血清中激素水平和精子发生及成熟过程,干扰下丘脑-垂体-睾丸轴的生理平衡,导致雄性生殖功能的降低。
Objective To study the combined toxic effects of carbaryl and fenvalerate on testicular lipid peroxidation, sperm parameters,the level of serum hormone and other effects in male rats. Methods According to 2x2 factorial analysis, forty healthy and clean adult male SD rats were randomly divided into four groups,including one control group （given corn oil） and three experimental groups：carbaryl （1/50 LD50, 11.2 mg/kg, dissolved in coin oil）,fenvalerate （1/50 LD50,9.02 mg/kg, dissolved in coin oil） and carbaryl＋fenvalerate （11.2 mg/kg ＋ 9.02 mg/kg, dissolved in coin oil）, 10 rats in each group, the treatment was conducted through gavage, once a day ,for eight consecutive weeks. Body weight gain,testis and epididymis organ weights were determined and the organ coefficients were counted. Sperm parameters were determined （sperm counts,the spermatozoon survival rate and the rate of the sperm deformation）. The activity of lipid peroxidation SOD, GSH, GST and MDA in testis homogenate were measured by the spectrophotometric method. The testosterone ,LH and FSH levels were determined in the serum by radioimmunoassay. Results There was no interaction between carbaryl and fenvalerate on rat body weight gain, organ body weight ratios of testis and epididymis （P〉0.05）. There was antagonism between earbaryl and fenvalerate on the spermatozoon survival rate and the rate of the sperm deformation. There was no interaction between carbaryl and fenvalerate on the sperm counts. There was antagonism between carbaryl and fenvalerate on the activity of SOD and MDA in testicle （P〈0.01, P〈0.05）. There was no interaction between carbaryl and fenvalerate on the activity of GST and GSH in testicle（P〉0.05 ）. There was antagonism between carbaryl and fenvalerate on the testosterone, LH and FSHI evels in the serum （P〈0.05,P〈0.01）. Conclusion Carbaryl combined with fenvalerate can cause obvious toxic effects on reproductive function in male rats. The change of testosterone biosynthetic enzymes and the levels of T in serum and sperm maturation process and disordered physiologic balances of hypothalamic-pituitary-testis axis may be the key factors.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第12期1072-1075,共4页
Journal of Environment and Health
基金
宁夏自然科学基金(NZ1099)
关键词
西维因
氰戊菊酯
联合染毒
生殖毒性
Carbaryl
Fenvalerate
Combined exposure
Reproductive toxicity
