摘要
目的了解福建省手足口病患者中CVB5(Coxsackie virus B5)病毒的变异及进化特征。方法采集2010年福建省手足口病例呼吸道标本,经RD细胞分离肠道病毒。病毒分离上清用于RNA提取并经RT-PCR法鉴定病毒型别。对鉴定为其他肠道病毒(非EV71或CVA16)的病毒,扩增病毒完整VP1区,扩增产物经克隆、筛选后测序。应用Mega软件对病毒VP1序列进行比较和种系进化分析。结果从2010年福建省手足口病患者中扩增得到6株完整的VP1区序列,序列比对证实有4株CVB5病毒,其他2株为Echo9病毒。序列差异比较表明,分离自福建省的CVB5病毒一致性程度较高,在种系进化上处于独立的进化分支,而与国内其他省份或其他国家的CVB5病毒分离株比较则有较大差异。结论 2010年福建省手足口病患者中存在CVB5病毒的感染,病毒与既往其它省份分离株有较大差异,提示CVB5病毒在福建省具有独特的传播链。
The aim was to explore the variance and phylogenetic features of Coxsackie virus B5（CVB5） isolated from hand-foot-mouth disease（HFMD）patients in 2010 in Fujian,China.The respiratory specimen from HFMD patients were collected and cultured on RD cells for viral recovery.Viral RNA was extracted form the supernatant of culture and used for viral typing by reverse-transcript PCR.The whole VP1 region of the CVB5 isolates was obtained by RT-PCR,and followed by cloning into plasmid and sequencing.The variance and phylogenetic relation between CVB5 isolates from Fujian and that from other provinces and counties were calculated by using Mega software.It was found that of 6 viral isolates from Fujian Province in 2010,four were identified as CVB5 and the others were Echo9.The sequence analysis indicated that the CVB5 isolates from Fujian shared higher identity and formed a distinct branch on the phylogenetic tree,whereas showed more difference when compared with isolates from other province in China and from other countries.These results demonstrated that CVB5 virus had emerged in HFMD patients in Fujian and the transmission chain of CVB5 virus in Fujian might be distinct from that from other provinces.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2011年第12期1086-1089,共4页
Chinese Journal of Zoonoses
基金
福建省自然科学基金项目(No.2010J01115)资助
关键词
手足口病
柯萨奇B5
种系分析
hand-foot-mouth disease
Coxsackie virus B5
phylogenetic analysis
