摘要
目的检测RANK在胃癌细胞系SGC-7901细胞中的表达,并进一步探讨细胞外调节蛋白激酶(ERK1/2)信号通路在RANKL诱导的胃癌细胞迁移中的作用。方法流式细胞术检测SGC-7901细胞表面RANK蛋白的表达;western-blot检测RANKL刺激后磷酸化ERK(p-ERK1/2)及ERK1/2的表达;Transwell法测定RANKL刺激后细胞迁移能力的改变。检测数据用x±s表示,采用SPSS16.0软件分析实验数据。结果 SGC-7901细胞表达RANK蛋白。RANKL诱导SGC-7901细胞迁移能力增强,RANK抑制剂rOPG抑制RANKL诱导的细胞迁移,RANKL刺激后,SGC-7901细胞p-ERK1/2表达升高,应用MEK/ERK的抑制剂PD98059显著抑制RANKL诱导的胃癌细胞SGC-7901迁移。结论胃癌细胞系SGC-7901细胞表达受体RANK,ERK1/2信号通路参与RANKL诱导的SGC-7901细胞迁移。
Objective There is no report about the role of RANKL/RANK pathway in gastric cancer cell migra- tion. The study was aimed to detect the expression of RANK in gastric cancer cell lines SGC-7901 cell, and furthermore, to explore the role of ERK1/2 in RANKL-induced gastric cancer cell migration. Methods Human gastric cancer cell lines SC-C-7901 obtained from the Type Culture Collection of the Chinese Academy of Sciences （Shanghai, China）. Detection of cell-surface expression of RANK protein was performed by fluorescence--activated cell sorting （FAC~）. Western blotting assayed the expression of phospho-ERK and ERK. Transwell assayed the migration of gastric cancer cells. Results Flu- orescence-activated cell sorting and western blotting showed that RANK is expressed in human gastric cancer cell line SGC-7901, and RANKL increased the migration of gastric cancer cell significantly, rOPG, the specific inhibitor of sRANKL could obviously block RANKL-induced gastric cancer cell migration. ERK1/2 was activated at 30 minutes after RANKL stimulated. PD98059, the MEK inhibitor obviously blocked RANKL-induced SGC-7901 ceils migration. Con- dusion This study provides for the first time that RANKL promoted RANK-expressing gastric cancer cell lines SCA2- 7901 cell migration. ERK1/2 was involved in RANKL-induced gastric cancer cell migration.
出处
《山西医药杂志（上半月）》
CAS
2011年第12期1177-1179,共3页
Shanxi Medical Journal
基金
国家青年科学基金(30700807)
辽宁省教育厅科技项目(2010225032)
