摘要
目的:以激光诱导棕色挪威(BN)大鼠脉络膜新生血管(CNV)为模型,观察健脾化浊法中药对CNV的组织病理学影响,探讨健脾化浊法中药对CNV的作用。方法:成年BN大鼠分为健脾组、三七组和空白组,光凝后3 d开始给药,30 d后处死动物,光镜下观察大鼠CNV组织病理学改变。结果:在CNV发生面积和最大中央厚度方面,健脾组均优于三七组和空白组。健脾组较之空白组在减少CNV面积上差异有非常显著的统计学意义(P<0.01);CNV厚度和面积三七组较之空白组差异均有统计学意义(P<0.05),健脾组较之三七组差异均有非常显著的统计学意义(P<0.01)。在光镜下,健脾组纤维血管组织增生比三七组和空白组明显减少,神经纤维层无显著改变。结论:健脾化浊法中药对CNV有一定的抑制作用。
Objective： To observe the histopathology and effect Jianpishenhuayuzhuo therapeutic on choroidal neovascularization（CNV）,Laser-induced CNV of brown norway（BN） rats as model.Methods： BN rats were divided into three groups.Medicine was given on the third days after photocoagulation,and rats were killed after 30 days.The histopathological changes were observed under light microscope.Results： In the area and maximum central thickness of CNV,reinforcing the spleen group was better than other groups.Compared reinforcing the spleen group with control group there was an absolutely significant difference（P0.01） in reducing the area of CNV,compared panax group with control group in thickness and area of CNV there were significant difference（P0.05）.Compared reinforcing the spleen group with panax group in the area and thickness of CNV there were absolutely significant differences（P0.01）.Under the light microscope,fibrovascular in reinforcing the spleen group was significantly reduced than other groups and nerve fiber layer had no significant changes.Conclusion： Thel Chinese medicine of reinforcing the spleen turn to muddy inhibits CNV.
出处
《现代医学》
2011年第6期627-630,共4页
Modern Medical Journal
基金
江苏省高校自然科学基础研究项目(07KJB36008)
关键词
健脾化浊法
脉络膜新生血管
组织病理学
大鼠
Jianpishenhuayuzhuo therapeutic
choroidal neovascularization
histopathology
rats
