摘要
目的观察复方血栓通对人视网膜色素上皮（RPE）细胞（CRL-2302）增殖、移行以及表达血管内皮生长因子（VEGF）和基质金属蛋白酶2（MMP-2）的影响,探讨其作用途径及机制。方法复方血栓通作用于CRL-2302细胞,采用MTS比色法,观察细胞增殖的情况;Transwell小室检测细胞移行的影响;Western blot和实时荧光定量RT-PCR方法分别检测细胞VEGF、MMP-2蛋白和mRNA的表达。结果 MTS比色法显示,一定浓度的复方血栓通胶囊（1.562 5~200 g/L）对VEGF诱导的CRL-2302细胞增殖具有抑制作用;Transwell小室实验显示,复方血栓通胶囊浓度为0,3.125,6.25和12.5 g/L时CRL-2302细胞移行数分别为183.67±13.32,145.67±14.57,87.33±29.14和34.67±7.51;3.125,6.25和12.5 g/L浓度的复方血栓通胶囊使CRL-2302细胞VEGF、MMP-2蛋白和mRNA的表达均显著降低。结论复方血栓通胶囊能够抑制RPE细胞的增殖、移行,这可能是其抑制RPE细胞参与血管生成的途径;其机制与抑制VEGF和MMP-2表达密切相关。
Objective It is to observe the influence of Complex Xueshuantong on proliferation,migration,and expression of vascular endothelial growth factor（VEGF） and matrix metalloproteinase 2（MMP-2） of human retinal pigment epithelial（RPE） cell line（CRL-2302） cells in vitro,in order to understand the action pathway and mechanism.Methods MTS method was used to access the effect of Complex Xueshuantong on the proliferation of CRL-2302 cells induced by VEGF.Transwell insert was used to detect the migration of CRL-2302 cells.Western Blot and real-time quantitative reverse transcription polymerase chain reaction（RT-PCR） were used to detect the expression of VEGF,matrix metalloproteinase 2（MMP-2） proteins and mRNA of CRL-2302 cells.Results The proliferation of CRL-2302 cells induced by VEGF was inhibited by Complex Xueshuantong at concentrations of from 1.562 5 to 200 g/L.When the concentration of Complex Xueshuantong was 0,3.125,6.25,and 12.5 g/L,cells number that migrated to under the Transwell membrane was 183.67±13.32,145.67±14.57,87.33±29.14,and 34.67±7.51 respectively.At concentrations of 3.125,6.25,and 12.5 g/L,Complex Xueshuantong could inhibit the expression of VEGF and MMP-2 proteins and mRNA of CRL-2302 cells.Conclusion Complex Xueshuantong could inhibit CNV concerned with CRL-2302 cells through the path of suppression of proliferation,migration of the cells.The inhibition of the expression of VEGF and MMP-2 proteins and mRNA of CRL-2302 cells may be one of the important mechanisms.
出处
《现代中西医结合杂志》
CAS
2010年第33期4241-4245,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
上海市卫生局中医药重大课题资助项目(2006ZD01)
关键词
复方血栓通
视网膜色素上皮细胞
增殖
移行
脉络膜新生血管
血管内皮生长因子
基质金属蛋白酶2
Complex Xueshuantong
retinal pigment epithelial（RPE） cell
proliferation
migration
choroidal neovascularization
vascular endothelial growth factor（VEGF）
matrix metalloproteinase 2（MMP-2）
