摘要
目的观察电针联合康复训练对脊髓损伤（SCI）大鼠脑源性神经营养因子（BDNF）及其受体酪氨酸激酶B（TrkB）表达的影响。方法选取96只成年SD雌性大鼠，采用脊髓切割损伤法制作右侧脊髓半横断损伤模型。将制模成功大鼠随机分为电针组、康复训练组、联合治疗组及模型组。于制模后第3天各组大鼠按既定方案给予相应干预，其中电针组、康复训练组分别给予电针督脉穴位或康复训练，联合治疗组于康复训练后辅以电针刺激。每周均对各组大鼠进行BBB运动功能评分；于制模后第4周及第8周时每组分别取12只大鼠处死，采用免疫组化法、PCR及RT-PCR技术检测各组大鼠受损脊髓BDNF及TrkB表达情况。结果各组大鼠BBB评分、BDNF及其受体TrkB表达均随时间延长逐渐增加，其中电针组、康复训练组及联合治疗组上述指标在制模后第4周、第8周时均明显优于模型组（P〈0．05），同时联合治疗组也显著优于电针组及康复训练组（P〈0．05）；电针组及康复训练组的上述指标组间差异均无统计学意义（P〉0．05）。结论电针督脉穴位联合康复训练治疗SCI大鼠具有协同疗效，能进一步加速大鼠运动功能恢复，其治疗机制可能与上调受损脊髓BDNF及其受体TrkB表达有关。
Objective To observe the changes of expressions of brain-derived neurotrophic factor （BDNF） and its receptor tyrosine kinase B （TrkB） and in rats with hemitranseetional spinal cord injury （SCI） after eleetroaeupuncture on Du Meridian and rehabilitation training. Methods The animal model of acute hemitransectional lesion at the right half of Tll spinal cord was established in 96 adult female rats, which were then divided randomly into an electroaeupuneture group, a rehabilitation training group, an electroacupuncture combined with rehabilitation training group and a control group. All the groups received treatment on the 3rd d after operation. The electroacupuncture group and rehabilitation training group were given electroacupuncture on points of Du Meridian and rehabilitation training, respectively, and the combined group was given Du Meridian electroacupuncture in addition to rehabilitation training. Basso-Beattie-Bresnahan （BBB） scale was used to eval- uate motor function every week. Twelve rats of each group were sacrificed 4 and 8 weeks after operation, respectively, and their spinal cord tissues were extracted. The polymerase chain reaction （PCR） , reverse transcrip- tion-polymerase chain reaction （RT-PCR） and immunohistochemical techniques were used to detect the expressions of BDNF and TrkB. Results BBB grade increased gradually as time went on. There were significant differences between control group and other groups at the same time point （ P 〈 0.05 ）. The scores increased ob- viously in eleetroaeupuneture combined with rehabilitation training group compared with electroacupuneture group and rehabilitation training group（ P 〈 0.05 ） . The result of immunohistochemical observation and RT-PCR also showed that there were significant differences of expressions of BDNF and TrkB among control group and other groups at the same time （P 〈 0.05 ）. The effects in electroaeupuneture combined with rehabilitation training group were much more obvious than those in electroacupuncture group and rehabilitation training group （ P 〈 0.05 ） , but there was no significant difference between electroacupuneture group and rehabilitation training group （P 〉 0. 05 ）. Conclusions Electroacupuncture on Du Meridian combined with rehabilitation training had synergic effect on rat＇s SCI, and could obviously improve the restoration of rat＇s motor function; the mechanism maybe related to the upregulation of expressions of BDNF and TrkB.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2011年第12期885-890,共6页
Chinese Journal of Physical Medicine and Rehabilitation
基金
基金项目：山东省中医药科技发展计划项目（2009-118）
关键词
脊髓损伤
脑源性神经营养因子
电针疗法
康复训练
Spinal cord injury
Brain-derived neurotrophic factor
Tyrosine kinase B
Electroacupuncture
Rehabilitation training
