摘要
对贵州省6个规模养猪场圈舍内外空气、污水、粪便和土壤样本进行细菌总数测定,并对不同环境样本中分离的大肠杆菌、沙门氏菌、葡萄球菌和链球菌进行药敏试验,以了解规模猪场环境细菌污染状况及主要病原菌的耐药性。结果显示,猪场环境细菌污染严重,其总数均一定程度地超出国家规定的卫生标准。其中,粪便样本含菌量最高,其次为污水、土壤样本,且粪便、污水和土壤样本细菌含量显著高于空气样本(0.01<P≤0.05);圈舍内空气中细菌浓度远远高于舍外细菌含量(0.01<P≤0.05)。药敏试验结果表明,猪场环境分离鉴定的细菌其耐药率较高。青霉素、四环素、洁霉素等传统抗生素已无明显抑菌效果,粪便、污水和土壤样本细菌耐药率均高于空气样本。
In the present study, total number and drug tolerance of bacteria （ Escherichia, Salmonella, Staphylococcus, Streptococcus） in air, sewage water, faecal and soil sample were investigated among six-scale pig farms. The result showed that bacteria amount in faecal ranked the first, followed by that in sewage water and soil, and the amount from abovementioned surrounding sites were higher than those in the air （0.01 〈 P≤ 0. 05 ）. Drug sensitive test proved that no remarkable effect of antibiotics on Penicillin, Tetracycline, and Lincomyeinthe was obtained. The tolerance rate of bacteria from sewage water and faecal were higher than that from the air. Therefore, the numbers of bacteria exceeded the requirement of national regulation for livestock and poultry farms, and the bacteria existed wide-spectrum drug tolerance.
出处
《山地农业生物学报》
2011年第6期554-557,共4页
Journal of Mountain Agriculture and Biology
基金
贵州省科技厅农业科技攻关项目[黔科合NY字(2009)3069]
贵州省科技厅农业科技攻关项目[黔科合NY字(2010)3072号]
关键词
猪场
细菌污染
耐药细菌
药敏试验
scaled farm
bacterial pollution
drug tolerance
tolerance test
