摘要
目的:建立高效液相色谱法测定山茱萸中科罗索酸的含量。方法:采用Inertsil ODS-3(150 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-水-磷酸(82∶18∶0.2),流速1.0 mL.min-1,检测波长205 nm,柱温30℃。结果:科罗索酸进样浓度在59.86～598.6μg.mL-1范围内与峰面积呈良好的线性关系,平均回收率(n=6)为98.7%(RSD=1.0%)。结论:该方法分离度好,专属性强,重复性好,简便易行,可用于山茱萸中科罗索酸的含量测定。
Objective：To establish an HPLC method for determination of corosolic acid in Corni Fructus.Methods：A Inertsil ODS-3（150 mm×4.6 mm,5 μm） column was adopted;The mobile phase consisted of methanol-distilled water-phosphoric acid（82∶ 18∶ 0.2） at a flow rate of 1.0 mL·min-1;The detection wavelenth was 205 nm,and the column temperature was 30 ℃.Results：The standard curves of corosolic acid shows a good linearity in the concentration range of 59.86-598.6 μg·mL-1,and the average recovery（n=6） was 98.7% with RSD of 1.0%.Conclusion：This method is simple,repeatable,and has good resolution and high selectivity.It can be used for the determination of corosolic acid in Corni Fructus.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第12期2217-2219,共3页
Chinese Journal of Pharmaceutical Analysis
