摘要
目的在肠道病毒71型(EV71)重组外壳蛋白VP1的基础上建立以化学发光为基础的IgM抗体检测技术,并与普通TMB显色酶联免疫吸附法比较,评价其临床应用前景。方法采用抗IgM单克隆抗体及辣根酶标记的重组VP1抗原,在此基础上建立EV71-IgM捕获法化学发光检测技术,并对比化学发光检测技术与普通酶联检测技术在45份EV71抗体阳性和30份阴性血清中的反应情况。结果化学发光法、普通酶联免疫吸附法、市售EV71-IgM检测试剂分别能与38份、19份、26份EV71抗体阳性血清发生阳性反应,灵敏度分别为84.4%、42.2%和57.8%;化学发光法灵敏度显著高于其他两种,差异有统计学意义(P<0.05);3种检测方法与阴性血清均无阳性反应。结论在EV71-VP1重组抗原的基础上建立的EV71-IgM捕获法化学发光检测技术具有很高的灵敏度,并有很好的临床应用前景。
Objective To establish the chemiluminescence immunoassay to detect IgM against Enterovirus 71 （EV71） based on recombinant protein of the capsid protein VP1 and evaluate its applications in clinic diagnosis. Methods The capture chemiluminescence immunoassay and routine ELISA method were established to detect IgM a gainst EV71 based on the recombinant VP1, and the chemiluminescence immunoassay were compared with ELISA and commercial kits in 45 sera of EV71 infected patients and 30 blood donors. Results The sensitivity of chemiluminescence immunoassay, routine ELISA, and commercial kits were 84.4%, 42.2 % and 57.8% respectively. The sensi- tivity of chemiluminescence immunoassay was significantly higher than that of EI3SA based on VPI and commercial kits （P〈0.05） ; There was no positive reaction with blood donor in the three methods. Conclusion Chemiluminescence immunoassay based on the recombinant VP1 antigen shows high sensitivity,and is promising in clinical diagnosis of EV71.
出处
《检验医学与临床》
CAS
2011年第24期2945-2946,2948,共3页
Laboratory Medicine and Clinic
基金
"十一五"国家科技重大专项平台建设(2009ZX10004-718)
