摘要
目的探讨时间分辨荧光免疫分析技术(TRFIA)乙型肝炎(下称乙肝)两对半定量检测的临床意义。方法用TRFIA与酶联免疫法(ELISA)对172例临床标本同时检测,并进行对比分析。同时对3 837份临床标本进行TRFIA检测结果的分析。结果两种方法检测结果经配对卡方检验差异无统计学意义(P>0.05)。3 837份临床标本其乙肝表面抗原(HBsAg)阳性率14.73%,乙肝表面抗体(抗-HBs)浓度大于100 mU/mL,占抗-HBs>10mU/mL,比例为49.58%。结论 TRFIA测定乙肝两对半具有灵敏、特异、定量、试剂稳定等优点,具有良好的应用价值。可同时定量检测进行正确分析,决定治疗的方案。
Objective To study the clinical significance of time-resolved fluorescence immunoassay（TRFIA） on the quantitative test of hepatitis B two fifty-fifty.Methods 172 clinical samples were tested and analyzed by TRFIA and enzyme-linked immunosorbent assay（ELISA）.Meanwhile,we analyzed the test result of 3 837 clinical samples by TRFIA.Results The results of the two methods showed no significant difference by paired chi-square test（P0.05）.The positive rate of hepatitis B surface antigen（HBsAg） from 3 837 clinical samples was 14.73%,and the concentration of hepatitis B surface antibody（HBsAb） 100 mU / mL accounted for 49.58% in HBsAb 10 mU/mL.Conclusion TRFIA test method is sensitive,specific,quantitative,reagents and stable,and is of good value for hepatitis B two fifty-fifty test.Quantitative test can be appropriately analyzed simultaneously to determine treatment strategy.
出处
《检验医学与临床》
CAS
2011年第8期902-904,共3页
Laboratory Medicine and Clinic
关键词
乙肝两对半
定量
分析
hepatitis B two fifty-fifty
quantitative
analysis
