摘要
目的:研究和解聚散方对单侧输尿管梗阻(unilateral ureteral obstruction,UUO)大鼠肾间质纤维化(renal inter-stitial fibrosis,RIF)、细胞外基质(extracellular matrix,ECM)、纤维连接蛋白(FN)和Ⅳ型胶原(ColⅣ)表达的影响。方法:采用UUO建立大鼠肾间质纤维化模型,随机分为模型组、贝那普利组、和解聚散方组,另设假手术组;术后7d、14d和21d分批取材,免疫组化法检测肾组织FN和ColⅣ的表达。结果:与模型组相比,和解聚散方治疗后7d、14d和21d,肾组织中的FN表达均减少(P<0.05),7d组ColⅣ的表达减少(P<0.05)。结论:和解聚散方对大鼠肾间质纤维化不同时间点FN和ColⅣ的下调,尤其是早期的下调作用,在一定程度上提示了和解聚散方的有效性和优越性,肾络聚散消长病机学说指导下的和解聚散法的正确性。
Objective：To study the effect of hejiejusan formula on expression of ECM, including fibronectin（FN） and Collagen Type Ⅳ（Col Ⅳ） in unilateral ureteral obstruction（UUO）-induced renal interstitial fibrosis（RIF） rats.Methods：RIF model rats were established by UUO. All the UUO rats randomly divided into model group, benazepril group, hejiejusan formula group. Healthy rats were chosen as the sham operation group. The rats were killed on the seventh, fourteenth and twenty-one days in batches respectively after UUO. The protein expression of FN and Col Ⅳ in kidney tissues were detected by Immunohistoche- mistry.Results：Compared with model group, the protein expression of FN in hejiejusan formula groups of three time points were all significantly decreased （P0.05）, but Col Ⅳ decreased only in 7 days group（P0.05）.Conclusion：Hejiejusan formula has the function of down-regulating the expression of ECM in RIF rats at three time points after UUO, especially early. It suggests to some extent, the effectiveness and superiority of hejiejusan formula, the correctness of hejiejusan method which is under the guidance of pathogenesis of shenluojusanxiaozhang theory.
出处
《中国中西医结合肾病杂志》
2011年第11期967-969,I0002,I0003,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
国家自然科学基金资助项目(No.30873223/H2704)
北京中医药大学校级自主项目(No.2009JYB22-JS076
2010JYB22-JS063)
教育部中医内科学重点实验室
卫生部肾病重点专科资助项目
关键词
和解聚散方
肾间质纤维化
细胞外基质
Hejiejusan formula Renal interstitial fibrosis Extracellular matrix
