摘要
目的探讨丹参酮ⅡA磺酸钠注射液是否对大鼠急性胰腺炎模型血清高迁移率族蛋白B1(HMGB1)有下调作用.方法复制重症急性胰腺炎组(A1组)及重症急性胰腺炎治疗组(A2组),轻症急性胰腺炎组(B1组)及轻症急性胰腺炎治疗组(B2组)动物模型,每组10只大鼠,A2、B2组于造模后3、27 h在大鼠后大腿外侧肌注丹参酮IIA磺酸钠注射液(4 mg/kg).于造模后12 h、24 h、48 h采血,采用酶联免疫法(ELISA)检测各组大鼠模型不同时间段血清HMGB1含量的变化.结果血清HMGB1测量值在各时间点A1组均高于A2组(P<0.05),B1组均高于B2组(P<0.05),差异具有统计学意义.结论丹参酮IIA磺酸钠注射液对大鼠急性胰腺炎模型血清HMGB1有拮抗作用.
Objective To explore the downregulating effect of Chinese medicine Sulfotanshinone Sodium on HMGB1 in rats with acute pancreatitis.Methods Forty rats were randomly divided into 4 groups：Severe acute pancreatitis（A1）group,Severe acute pancreatitis treatment（A2） group,mild acute pancreatitis（B1）group and mild acute pancreatitis treatment（B2）group.Rats in A2 and B2 groups were given injection of ulfotanshinone Sodium（4 mg/kg）3 and 27 hours after establishment of acute pancreatitis.The blood samples of rats in 4 groups were collected at 12 h,24 h and 48 h after establishment of acute pancreatitis.The serum levels of HMGB1 were detected by enzyme league immune method（ELISA）.Result The serum levels of HMGB1 in group A1 were higher than the group A2 at different time points（P 0.05）,in group B1 were higher than the group B2 at different time points（P 0.05）.Conclusion Chinese medicine Sulfotanshinone Sodium has downregulating effect on HMGB1 in rats with acute pancreatitis.
出处
《昆明医科大学学报》
CAS
2012年第11期32-34,37,共4页
Journal of Kunming Medical University
基金
云南省卫生厅内设研究机构基金资助项目(2011WS0097)
