摘要
采用放射型根瘤杆菌植酸酶粗酶液水解麸皮中的植酸,研究其脱磷的最适条件。依据单因素试验和正交试验获得脱磷的最优条件为加酶量750U/g、底物质量浓度40mg/mL、温度45℃、pH7、时间5h。最优条件下,麸皮中植酸的水解率在5h达最大,为81.50%。
Crude phytase solution separated from Agrobacterium radiobacter fermentation broth was used to hydrolyze wheat bran for phytic acid dephosphorization.Using one-factor-at-a-time combined with orthogonal array design method,such process conditions as enzyme amount,substrate concentration,pH,temperature and hydrolysis time were optimized to be 750 U/g,40 mg/mL,7,45 ℃,and 5 h,respectively.Under the optimized conditions,the hydrolysis rate of phytic acid was up to 81.50%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第22期105-108,共4页
Food Science
基金
江苏省高校自然科学研究面上项目(10KJD180006)
徐州工程学院科研基金青年课题(XKY2009122)
徐州工程学院科研基金培育课题(XKY2010112)
关键词
植酸酶
脱磷
麸皮
phytase
dephosphorization
wheat bran
