摘要
目的：研究人骨肉瘤中P53蛋白表达及P53基因突变及其与预后的关系。方法：免疫组织化学染色观察人骨肉瘤中p53蛋白表达，银集PCR－SSCP法检测常规石蜡包埋人骨肉瘤组织中p53基因5～8外显子点突变状态。预后分析用Kaplan－Meier生存曲线及Logrank时序检验。结果：人骨肉瘤中p53蛋白阳性率为29．17％（28／96），SSCP检测p53基因5～8外显子点突变率为23．96％（23／96），P53蛋白及SSCP阳性患者与其阴性患者间总生存率比较在统计学上差异无显著性（P＞0．05），但在第1年内P53蛋白阳性及SSCP阳性患者的生存率明显低于其阴性者（P＜0．05）。P53基因点突变与P53蛋白表达的检测阳性符合率为82．14％，以第7外显子出现突变的频率最高。结论：p53蛋白表达及点突变检测有可能成为判断骨肉瘤恶性程度、转移潜能及预后的指标；p53蛋白表达在一定程度上可以反映p53基因的点突变。
Purpose To investigate the expression and mutation of p53 gene in human osteosarcoma andtheir relationship to prognosis. Methods The expression of p53 protein was detected by immunohistochemicalstain. The point mutation of p53 gene was detected by silver staining PCR-SSCP in paraffin-embedded osteosar-coma. The prognosis was analyzed by Kaplan-Meter survival curve and Logrank test. Results The positive rateof p53 protein expression and point mutation were 29. 17% and 23. 96 % individully. The positive coincidencerate between p53 gene mutation and protein expression was 82. 14 %, mutation in exon 7 was highest among the5, 6, 7, 8 exon. p53 protein positive and SSCP positive patients had a worse out come in the first year than theirnegative conterparts, but there is no difference overall(P >0. 05 ). Conclusion The protein expression and mu-tation of p53 gene of osteosarcoma might be a predict variable in judgement of malignancy, metastasis and prog-nosis. And, in some extent, the p53 protein expression can reflect the p53 gene mutation aproximately.
出处
《临床与实验病理学杂志》
CAS
CSCD
1999年第6期474-476,I067,共4页
Chinese Journal of Clinical and Experimental Pathology
