摘要
从土壤中分离筛选到一株放线菌菌株XWC8,经微波-硫酸二乙酯复合诱变,获得一株高产纤维素酶突变株XWC8-d。文章运用正交试验法对突变株XWC8-d进行固态发酵条件研究,确定了菌株XWC8-d的最优产酶条件,即最适的单一碳源为麦麸(15g),以NH4NO3(0.3g)为单一氮源,固液比为1∶2.3,25℃下培养96h。在优化条件下,XWC8-d的天然纤维素酶活为26.79μmol/min,CMC酶活为26.27μmol/min,FPA酶活为26.94μmol/min,分别比出发菌株XWC8提高了189%、102%和96.8%。
A cellulose-decomposing Actinomyces strain was isolated from natural habitats, which named XWC8. A high cellulase-producing strain XWC8-d derived from XWC8 was obtained after the mutagenesis by DES and microwave treatment. Orthogonal experiment was used to evaluate the cultural conditions for cellulase production of XWC8-d by studying its solid-state fermentation conditions. The results show that the optimal conditions for cellulase production of XWC8-d are as follows.. the bran（15 g） and NH4NO3 （0.3 g） are used as single carbon source and single nitrogen source respectively, the mass ratio of solid material to water is 1 ： 2. 3, and the culture is conducted at 25 ℃ for 96 h. Under these optimized conditions, the eellulase, CMCase and FPase activities of XWC8-d reach 26.79 μmol/min, 26.27 μmol/min and 26.94 μmol/min respectively, which are 189%, 102% and 96.8% higher than those of XWC8 respectively.
出处
《合肥工业大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第12期1873-1876,共4页
Journal of Hefei University of Technology：Natural Science
基金
安徽省年度重点科研计划资助项目(09020303079)
关键词
放线菌属
纤维素酶活
固态发酵
诱变
Actinomyces
cellulase activity
solid-state fermentation
mutagenesis
