摘要
目的：探讨双黄连注射液２ 种指标成分含量的测定方法，控制其质量。方法：应用甲醇水溶液（ 磷酸调ｐＨ至２ ．７） 为流动相，在ＯＤＳＣ１８ 柱上梯度洗脱，外标法定量，绿原酸和黄芩苷２ 种指标成分含量可以同时定量。结果：３ 批样品中氯原酸的含量分别为０ ．７０ ％ ，０ ．７４ ％ 和０ ．７５ ％ （ｍｇｍｇ） ，黄芩苷的含量分别为１０ ．６７ ％ ，１０ ．２５ ％ 和１１ ．３８ ％ （ｍｇｍｇ） 。结论：该方法简便、快速、灵敏，适合于双黄连粉针剂的质量控制。
OBJECTIVE:To develope a method for the determination of chlorogenic acid and baicalin in Shuanghuanglian injection,to contral its quality.METHOD:The colum was packed with ODS C 18 (150mm×4.6mm).Methanol water(pH 2.7) was used as mobile phase.Chlorogenic acid and baicalin were separated by HPLC with grade elution.RESULTS:The content of chlorogenic acid in Shuanghuanglian injection was 0.70%～0.75%(mg/mg),and that of baicalin was 10.25%～11.38%(mg/mg).CONCLUSION:The method was rapid convenient and accurate,and is suitable for the quality control of Shuanghuanglian injection.
出处
《中国现代应用药学》
CAS
CSCD
1999年第6期47-49,共3页
Chinese Journal of Modern Applied Pharmacy
关键词
双黄连
绿原酸
黄芩苷
高效液相色谱法
Shuanghuanglian injection,chlorogenic acid,baicalin,HPLC,gradient elution
