摘要
探讨骨形成蛋白（BMP）对人骨肉瘤细胞的作用及意义．方法：将重组人BMPZ基因的反义核酸逆转录病毒表达载体PDOR－AB用脂质体导入BMP表达阳性的骨肉瘤细胞OS－99OI，观察骨肉瘤细胞OS－99OI的BMP表达、增殖活性、肿瘤抑制基因＂m23的表达、细胞周期和电镜下形态改变．结果：转染后，OS－99o1细胞中内源性的BMP表达产物明显下降；增殖活性明显下降；肿瘤转移抑制基因＂m23表达未见改变；细胞周期分析表明：GI期细胞比例上升，S期比例下降；电镜观察可见细胞质中溶酶体增多，染色质有断裂现象．结论：反义核酸技术阻断BMPZ在OS－99o1细胞中的表达后，肿瘤细胞的增殖活性降低，证实骨肉瘤细胞中BMP的表达增加了肿瘤细胞的增殖活性．
AIM: To further understand the role of BMP2 ex-pression in osteosarcoma cell line (OS-99O1). METHODS:This recombinant vector encording antisense cDNA of BMP2RNA (pDOR-AB) was transduced into osteosarcoma cell lineOS-99o1 by lipofectin and the biological activity of transfect-ed OS-99Ol cells was analyzed by immunohistochemistry forthe expression of introcellular BMP2, PCNA, and nm23gene, and by FCM for the morphological changes- RE-SULTS: Both the expression of BMP2 and proliferating activ-ity of transfected cells were obviously decreased and Gl phaseof transfected cells increased while S phase reduced when ob-served by FCM. There was no difference in nm23 gene ex-pression before and after transfection. The electronmi-croscopic observation showed increased lysosome chromatinchange in some cells. CONCLUSION: The results of ourstudy demonstrate that the expression of antisense BMP2RNA decreases the proliferating activity of OS-99O1 cells andBMP2 may play an important role in the biological activity ofosteosarcoma cells.
出处
《第四军医大学学报》
1999年第12期S123-S125,共3页
Journal of the Fourth Military Medical University
关键词
骨形成蛋白
骨肉瘤
反义RNA
细胞转染
bone morphogenetic protein
osteosarcoma
antisense RNA
cell line
transfection
