摘要
建立了反相高效液相色谱法(RP-HPLC)分离肝源性磷脂酰胆碱(PC)分子种的有效方法。考察了流动相中有机溶剂的种类、配比及流速对分离肝源性PC的影响。研究发现,在以甲醇为主的流动相中加入正己烷和醋酸铵有利于肝源性PC分子种的分离;甲醇-乙腈-水梯度洗脱不适于分离肝源性PC分子种。结果表明,采用Kromasil C18色谱柱(4.6 mm×200 mm,5μm),以甲醇-正己烷-0.05 mol/L醋酸铵-甘油(84∶6∶8∶0.6,体积比)为流动相,在流速1.0 mL/min、检测波长206 nm、柱温35℃的条件下,实现了肝源性PC各组分的分离。所建立的方法灵敏,重复性高,为进一步采用液质联用技术研究肝源性PC不同分子种的结构奠定基础。
In this study an effective method for separating phosphatidylcholine （PC） molecules from swine liver was developed by using reversed-phase high performance liquid chromatography. The effect of organic modifiers, the composition of mobile phase and the flow rate on PC molecules separation were also investigated. The results showed that the separation process benefited by adding hexane and ammonium acetate into the mobile phase with methanol as the dominant solvent. The method of methanol-acetonitrile-water gradient elution did not perform well in separating the PC molecules. The RP-HPLC method for separating PC molecules on Kromasil C18chromatographic column （4.6 mm ×200 mm, 5μm） was established with good sensitivity and reproducibility, by using methanol-hexane-0.05 mol/L ammonium ace- tate-glycerol （ 84： 6： 8： 0.6, V/V） as the mobile phase at the flow rate of 1.0 mL/min, testing wavelength of 206 nm and column temperature of 35 ℃ This research lays a foundation for analyzing the structures of the PC molecules in the futtre
出处
《天然产物研究与开发》
CAS
CSCD
2011年第6期1144-1147,共4页
Natural Product Research and Development
关键词
高效液相色谱法
分离
肝源性磷脂酰胆碱
high performance liquid chromatography
separation and purification
phosphatidylcholine from swine liver
