摘要
目的:检测GST- π基因在卵巢癌组织中的表达,探讨GST- π与卵巢癌癌变的关系。方法:采用免疫组化和RT- PCR 方法,检测正常卵巢及卵巢良、恶性肿瘤组织中GST- π m RNA 及蛋白水平的变化。结果:GST-π在卵巢组织中的表达与肿瘤的进展密切相关,即正常组织中GST- π几乎不表达,良性肿瘤中水平略有升高,交界性肿瘤多为低、中度表达,但阳性率明显升高,接近卵巢癌组织的水平。癌变组织则为中、高度表达且随临床分期的发展而逐渐增高。免疫组化与RT- PCR 的结果符合率较高,但以RT- PCR 法较为灵敏。结论:GST- π是与卵巢癌发生、发展密切相关的标志酶,它的表达异常与卵巢癌的预后有关。
Objective: GST-π gene expression was measured in ovarian cancer and its role in carcinogenesis was also discussed. Methods: Immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) were used to determine the expression level of GST-π in 139 ovarian biopsy sections. Results: The expression level of GST-π was related to the development of ovarian tumor and an increasing tendency was observed in the normal tissue, benign tumor, borderline malignancy and malignant tumor. The positive rates obtained from immunohistochemical staining were 0%, 2.5%, 71%, 75.8% respectively, while the same from RT-PCR were 0%, 5%, 71%, 85.5% respectively. Therefore, the results indicated that RT-PCR was more simple, rapid, specific and sensitive than immunohistochemical method. Conclusion:Glutathione S-Transferase-π might play a key role in the development of human ovarian cancer and relates with the prognosis of cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1999年第11期836-838,共3页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金资助! ( 编号 39570733)
