摘要
目的探讨多药耐药基因GST—πmRNA在胃癌组织中的表达及临床意义。方法采用逆转录-聚合酶链反应（reverse transcription—polymerasec haint eaction．RT-PCR）技术检测53例新鲜胃癌组织标本（实验组）和15例正常胃黏膜组织（对照组）中GST-π基因的表达．并分析其表达与胃癌病理特征之间的关系。结果①53例胃癌组织中GST-πmRNA的表达（0．56±0．27）要高于15倒正常胃黏膜组织的表达（0．18±0．13）（t=5．30．P〈0．001）。②GST-πmRNA的表达与胃癌的分化程度之间差异具有统计学意义（t=2．67．P=0．01），而与患者性别、肿瘤部位、大小之间、是否淋巴结转移差异无统计学显著性意义。结论①GST-πmRNA的高表达可能是胃癌产生多药耐药的机制之一；②GST—π的检测对临床制定化疗方案有一定指导意义。
Objective To investigate the expression levels of GST-π mRNA in patients with gastric cancer and clinical signifi- cance. Methods RT-PCR method was used to detect the expression levels of GST-II mRNA in fresh gastric cancer 53 cases （experiment group） and 15 cases of normal gastric mucosa tissue（eontrol group）,analyzed the relationship between the expression of GST-π and the clinicopathologic parameters. Results （1） The expression level of GST-nmRNA （0.56 ± 0. 27） was significantly higher in gastric cancer tissues than that in normal tissues （0. 18±0.13） （t=5.30,P-（0. 001）. QThe expression of GST-π intensity was related to differentiation degree（t= 2.67. P= 0.01）, had nothing to do with other clinico- pathologic parameters. Conclusion OThe over-expression levels of GST-πm RNA would underline the muhidrug resistance of gastric cancer;（2）The detection of expression can be looked as an importance method for judging the prognosis in gastric cancer.
出处
《现代检验医学杂志》
CAS
2012年第5期41-44,共4页
Journal of Modern Laboratory Medicine
关键词
胃癌
多药耐药
GST-Π基因
表达
gastric cancer
muti-drug resistance
GST-πgene
expression
