摘要
目的：探讨Ｈｅ－Ｎｅ激光对ＴＮＦ基因的调控作用。方法：用斑点杂交及Ｎｏｒｔｈｅｒｎ 杂交技术观测激光对肿瘤患者ＴＮＦ基因表达的影响，采用２４ｍ Ｗ Ｈｅ－Ｎｅ激光照射荷瘤小鼠及肿瘤患者脾区（瘤区），照射前后分别取脾细胞及外周血淋巴细胞，从中提取ＲＮＡ。通过双抗体夹心ＥＬＬＳＡ 法及受体吸收试验，观测激光照射对带瘤机体ＴＮＦ受体及ＴＮＦ活性的影响。结果：带瘤机体ＴＮＦｍ ＲＮＡ 及ＴＮＦ活性明显降低，血清及培养上清中可溶性ＴＮＦ受体（ｓＴＮＦＲ）的表达量增高。但经激光照射后ＴＮＦｍ ＲＮＡ 表达量显著增高，ＴＮＦ活性增强，而ｓＴＮＦＲ 含量减少，瘤块明显减小。照射前后结果比较，差异有高度显著性（Ｐ＜ ０．０１）。结论：Ｈｅ－Ｎｅ激光照射具有促进ＴＮＦｍ ＲＮＡ
Objective:To study the influence of He-Ne laser on TNF gene expression.Methods:24mW He Ne Laser was applied to irradiate spleen region with tumor in patients and mice.Spleen cell and lymphocyte in tumor body were obtained before and after irradiation, and RNA was drawn.We used spot blot hybridization and northern blot hybridization technique to observe the effect of laser on TNF gene expression in patients with tumor.Results:TNFmRNA was lower in tumor body.After laser irradiation,TNFmRNA was signifcantly raised( P <0 01),difference was notable. Conclusion:He Ne laser may promote TNFmRNA expression.
出处
《滨州医学院学报》
1999年第6期521-525,共5页
Journal of Binzhou Medical University
基金
山东省教委资助课题
