摘要
Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the development and applications of this bioprocess. In this article, mutiple ways for breeding of microbial strains containing PAL were reviewed in details, including direction screening, enrichment culture technique for isolating strains from nature sources, phsical and chemical mutagenesis for strain improvement, PAL molecular clone and genetic engineering, etc. The significance of protoplast techniques for breeding of Rhodotorula strains containing PAL was analysed and discussed.
Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the development and applications of this bioprocess. In this article, mutiple ways for breeding of microbial strains containing PAL were reviewed in details, including direction screening, enrichment culture technique for isolating strains from nature sources, phsical and chemical mutagenesis for strain improvement, PAL molecular clone and genetic engineering, etc. The significance of protoplast techniques for breeding of Rhodotorula strains containing PAL was analysed and discussed.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第6期643-650,共8页
Chinese Journal of Applied and Environmental Biology
基金
中国科学院成都地奥科学基金!(DASF)
关键词
菌种选育
苯丙氨酸解氨酶
肉桂酸生物转化
L-phe
strain breeding
L
phenylalanine ammonia lyase(PAL)
trans cinnamic acid biotransformation
