摘要
应用异源双链泳动分析法（Ｈｅｔｅｒｏｕｄｕｐｌｅｘ ｍｏｂｉｌｉｔｙ ａｓｓａｙ，ＨＭＡ） ，对来自我国云南、四川、新疆、浙江、广东、福建、天津７ 省市７３ 份ＨＩＶ 感染者样品，进行了ＨＩＶ－ １ 膜蛋白（ｅｎｖ） 基因片段亚型分析。通过ＨＭＡ 能确定亚型的有７０ 份，占样品总数的９５－８９ ％ （７０／７３） 。其中Ａ 亚型２－８６ ％ （２／７０） ，Ｆ亚型２ ．８６ ％ （２／７０） ，泰国Ｂ 亚型１８ ．５８ ％ （１３／７０） ，欧美Ｂ 亚型８ ．５６ ％ （６／７０） ，Ｃ亚型３８ ．５７ ％ （２７／７０） ，Ｅ亚型２８－５７ ％ （２０／７０） 。为了检验结果的准确性和探讨３ 份样品不能用ＨＭＡ分型的原因，进一步对样品进行序列测定和系统树分析，并将两者结果的相互比较，两者对ＨＩＶ－ １ 分型显示出高度的一致性，其亚型一致率达９５－８９ ％ （７０／７３） 。而３ 份样品不能确定亚型的原因主要是，其ｅｎｖ 基因区段与相应的参考亚型质粒毒株相比变异太大。这提示，为了提高ＨＭＡ分型的敏感性，应不断地选择代表性好的毒株构建参考亚型质粒。
We investigated the DNA fragment diversity of HIV-1 env gene by means of heteroduplex mobility assay (HMA) genotyping. Samples of 73 HIV-1 infected individuals from Yunnan, Sichuan, Xinjiang, Zhejiang, Guangdong, Fujian and Tianjing of our country were studied. The HMA had successfully genotyped 95.89% (70/73) of these samples as follows: 2.86% (2/70) subtype A, 2.86% (2/70) subtype F, 18.58% (13/70) subtype Thailand B, 8.56% (6/70) subtype USA B, 38.57% (27/70) subtype C and 28.57% (20/70) subtype E. In order to test the reliability of these results and analyse the reason of failure to genotyping three samples by HMA, these strains were sequenced and phylogenetically analysed, and the results showed a perfect concordance with the HMA, the concordance rate was 95.89% (70/73). Reason of being unable to genotype three samples by HMA is that they were highly divergent subtype as compared to related reference strains. It suggests that, to improve reliability of HMA, the representative reference subtype plasmid selection must be continuously revised to cover viral diversification.
出处
《病毒学报》
CAS
CSCD
北大核心
1999年第4期296-304,共9页
Chinese Journal of Virology
关键词
HIV-1
HMA
序列测定
系统树
基因亚型
AIDS
Human immunodeficiency virus type 1
Subtype
Heleroduplex mobility assay (HMA)
Sequencing
Phylogenetic tree
