摘要
目的：研究Ｐ物质（ＳＰ）对脾细胞免疫功能的调节作用。方法：采用３Ｈ－ＴｄＲ掺入法、放射免疫测定和流式细胞仪等方法，检测了ＳＰ对体外培养的大鼠脾细胞的增殖和分泌ＩＬ－２、ＩＬ－６、ＴＮＦ功能及Ｔ细胞表型表达的影响。结果：ＳＰ能明显刺激脾细胞的增殖，其中浓度为１×１０－ ４ｍ ｍ ｏｌ／Ｌ时作用最强；ＳＰ能促进Ｔ细胞ＣＤ分子的表达，使ＣＤ４＋ ／ＣＤ８＋ 升高；ＳＰ能显著促进脾细胞分泌ＩＬ－２，对脾细胞分泌ＩＬ－６具有抑制作用，而对分泌ＴＮＦ无作用。结论：证实了ＳＰ是具有广泛的免疫调节作用的一种神经肽。
Objective: To explore the effectsofsubstance P(SP) on im mune function ofspleniccells. Methods: Afterthe spleniccellsfrom ratswerecultured in vitro, theirproliferative activity was determ ined w ith incorporation of 3H-TdR, production ofcytokines detected by RIA and phenotype ofT lym phocytes investigated w ith FCM. Results: ①SPaugmented the proliferation ofsplenic cells to a peak of 1×10- 4 m m ol/L. ②SPprom oted the expression ofphenotypeofTlym phocytesto resultin theincreasein num ber ofCD+3 and CD+4 Tlym phocytes. ③SPsignificantly promotedthesecretion ofIL-2butdecreased thatofIL- 6 and did notaffectthatofTNFfrom the spleniccells. Conclusion: SPis a kind ofneuropeptide with high capacity ofimm unity regulation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第12期896-898,共3页
Journal of Third Military Medical University
基金
军队"九五"攻关课题!项目(96L042)
