摘要
目的 探讨抗独特型抗体对小鼠移植耐受的诱导作用。方法 以C5 7BL 6小鼠脾细胞免疫Balb c小鼠制备抗同种异品系抗体 (Ab1) ,Ab1交联KLH后 ,免疫Balb c小鼠诱导产生抗独特型多克隆抗体 (Ab2 ) ,以之为移植受体 ,观察Ab2对小鼠心肌移植耐受的诱导作用。结果 Ab1交联KLH和弗氏佐剂免疫可以有效诱导抗独特型抗体 (Ab2 ) ,和对照组相比 ,Ab2诱导组的小鼠移植物的存活时间明显延长。结论 移植前在受体体内诱导产生以移植物抗原为模拟抗原的抗独特型的抗体 。
Objective To investigate the induction effect of anti idiotypic antibodies on mice specific low immune reactive state.Methods Balb/c mice were vaccinated by C57BL/6 spleen cells to obtain antibodies (Ab1) , Ab1 was cross linked with KLH , Ab1 KLH and freund's adjuvant were used to induce anti idiotypic antibodies(Ab2) in Balb/c mice of transplantation recipient. Cardiac tissue of newborn C57BL/6 mice was transplanted to Balb/c mice. The survival of transplanted tissue and other rejection indexes was observed. Results The results showed that Ab1 KLH and freund's adjuvant can induce Ab2 effectively. Compared with control group , the mean survival time of cardiac tissue graft of Ab2 induction group was significantly longer, and other rejection indexes were significantly differently ( P <0.01).Conclusion Graft's antigens as initiation antigens, the method of anti idiotypic antibodies induction before transplantation can suppress rejection and prolong the survival time of graft effectively.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2002年第B06期7-9,19,共4页
Immunological Journal
基金
全军医药卫生科研基金资助项目 (98M10 9)
