摘要
目的探讨利多卡因（Ｌｉｄｏ）对内毒素（ＥＴ）激活的中性粒细胞（ＰＭＮ）致内皮细胞损伤的保护作用。方法利用体外培养的内皮细胞，建立ＥＴ－ＰＭＮ－血清（ＨＳ）致内皮细胞损伤的模型，选用原代培养的小牛主动脉内皮细胞，纯化后４～９代的细胞制成悬液，接种于培养板，随机分为三组：对照组（ＥＣ＋ＰＭＮ＋ＰＢＳ），损伤组（ＥＣ＋ＰＭＮ＋ＨＳ＋１０μｇ／ｍｌ ＥＴ）和 Ｌｉｄｏ组（ＥＣ＋ＰＭＮ＋ＨＳ＋１０μｇ／ｍｌＥＴ＋１０ｕｇ／ｍｌ Ｌｉｄｏ）；另设三组（１μｇ／ｍｌ、１０μｇ／ｍｌ和１００μｇ／ｍｌ Ｌｉｄｏ）比较不同浓度Ｌｉｄｏ对内皮细胞的增殖活力的影响；用比色法测定培养液中乳酸脱氢酶活性、丙二醛浓度，用ＭＴＴ法测定细胞增殖活力（ＭＴＴ ＯＤ值）。结果与对照组比较，两组细胞培养上清乳酸脱氢酶活性及丙二醛浓度明显增加（Ｐ＜０． ０５），ＰＭＮ与内皮细胞粘附率明显增加（Ｐ＜０． ０１），损伤组ＭＴＴ ＯＤ值明显降低（Ｐ＜０．０５）。与损伤组比较Ｌｉｄｏ组细胞培养上清乳酸脱氢酶活性及丙二醛浓度显著降低（Ｐ＜０．０５），低浓度（１μｇ／ｍｌ）和中浓度（１０μｇ／ｍｌ）Ｌｉｄｏ ＭＴＴ ＯＤ值明显增加（Ｐ＜０．０５）。结论一定浓度的利多?
Objective To determine if lidocaine (Lido) could protect endothelial cell(EC) from endotoxin (ET)-activated polymorphonuclear leukocytes (PMN) injury. Methods Using in vitro model of ET-PMN-human serum(HS), endothelial cells were collected from a newborn calf descending aorta and were purified to be cultured. The cultured endothelial cells were divided randomly into 3 groups:control group (EC+PMN + PBS), injure group (EC+PMN+HS+ 10μg/ml ET) and Lido group (EC+PMN +HS+10μg/ml ET+10μg/ml Lido). Additionally three different levels of Lido (1μg/ml, 10μg/ml and 100μg/ml) were selected. LDH activity and MDA level and the vitality of cell proliferation were measured with calorimetric assay. Results Compared with those in control group ,LDH activity,MDA level and the rate of adhension PMN to EC increased significantly (P<0.05)in injury group and Lido group (P<0. 05 or 0.01), the OD value decreased markedly only in injury group (P<0. 05). The LDH activity,MDA level and rate of adhension PMN to EC decreased obviously and the OD value increased significantly as compared with those in injury group (P<0.05). Conclusion Lidocaine at a certain level can be helpful for attenuation of EC damage induced by ETactivated PMN.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
1999年第12期736-738,共3页
Chinese Journal of Anesthesiology
