摘要
目的：研究胶质瘤中ｐ５３ 、ｂｃｌ２ 、ＭＤＭ２ 表达与细胞增殖和凋亡的关系。方法：对４８ 例胶质瘤用免疫组化法检测ｐ５３、ｂｃｌ２、ＭＤＭ２ 蛋白表达；以Ｋｉ６７ 标记指数和ＡｇＮＯＲ 计数检测其增殖活性；用ＴＵＮＥＬ 法检测细胞凋亡。结果：ｐ５３、ｂｃｌ２、ＭＤＭ２ 蛋白表达阳性率分别为４７－９ ％ 、３５－８ ％ 及１２－５ ％ ，细胞增殖活性随肿瘤ｐ５３ 、ｂｃｌ２、ＭＤＭ２ 表达水平增高而增高，细胞凋亡则相反。结论：ｐ５３、ｂｃｌ２、ＭＤＭ２ 蛋白过表达与细胞增殖失控、凋亡抑制关系密切，在胶质瘤恶性进展中起重要作用。
Purpose To study the expression of p53、bcl 2 and MDM2 as well as their relationship with proliferation activity and apoptosis in gliomas. Methods p53、bcl 2 and MDM2 expression in 48 cases of gliomas were detected by immunohistochemical staining. Cell proliferation activity of gliomas was evaluated by Ki 67 labeling index ( LI ) and number of AgNOR and cell apoptosis was examined with TUNEL method. Results The positive expression rate of p53、bcl 2 and MDM2 was 47 9%, 35 8% and 12 5% respectively. Proliferation activity of gliomas was elevated with the increasing level of p53、bcl 2 and MDM2 expression, which was opposite to apoptosis. Conclusion Over expression of p53、bcl 2 and MDM2 were closely related to uncontrolled cell proliferation and inhibition of apoptosis in gliomas. They play an important role in malignant progression of gliomas.
出处
《临床与实验病理学杂志》
CAS
CSCD
1999年第5期402-404,I060,共3页
Chinese Journal of Clinical and Experimental Pathology
关键词
神经胶质瘤
P53蛋白
BCL-2蛋白
MDM2
gliomas
p53 protein
bcl 2 protein
MDM2 protein
cell proliferation activity
apoptosis
