摘要
目的探讨白藜芦醇(RES)对肠缺血再灌注致肠黏膜损伤大鼠的保护作用及其可能机制。方法成年雄性SD大鼠24只随机分为假手术组、缺血再灌注损伤组、RES治疗组。假手术组仅分离肠系膜上动脉(SMA)而根部不夹闭。肠缺血再灌注损伤组和RES治疗组均用无损伤血管夹夹闭SMA根部,分别立即经阴茎背静脉注射9 g.L-1盐水、RES(20 mg.kg-1),45 min后放松血管夹形成再灌注。各组大鼠均于制模后6 h采集静脉血和回肠标本。检测血清二胺氧化酶(DAO)及小肠脂肪酸结合蛋白(IF-ABP)水平,应用原位末端转移酶标记法检测肠黏膜上皮细胞凋亡率,HE染色法观察肠组织病理损伤情况。结果肠缺血再灌注6h后,RES治疗组DAO及IFABP水平与肠缺血再灌注损伤组比较显著减少[DAO:(1 650±1 150)U.L-1 vs(2 920±1 520)U.L-1;IFABP:(845.12±123.86)μg.L-1 vs(1 443.76±174.62)μg.L-1,Pa<0.05],但二组较假手术组[(630±150)U.L-1,(26.76±4.86)μg.L-1)]均显著增加(Pa<0.05)。假手术组大鼠肠绒毛顶部、固有层和黏膜下层只有少量散在分布的凋亡细胞,缺血再灌注损伤组大鼠肠黏膜凋亡阳性细胞数量较假手术组明显增加[(66.63±1.71)%vs(9.60±1.76)%,P<0.05],分布范围从绒毛顶部扩大到中、底部,固有层和黏膜下层,细胞凋亡亦明显加重;RES组大鼠肠黏膜细胞凋亡率[(46.72±1.50)%]明显低于缺血再灌注损伤组(P<0.05)。结论 RES对肠缺血再灌注损伤具有保护作用,其机制可能与其抑制肠黏膜上皮细胞凋亡有关。
Objective To study the protective effect of resveratrol（RES） on intestinal mucosal barrier in ischemia-reperfusion rats and explore the possible mechanism. Methods Twenty-four SD rats were randomly divided into the sham-operation group,ischemia-reperfusion group and RES-treated（RES） group.In sham-operation group,the superior mesenteric artery was separated but not clamped.In RES group and ischemia-reperfusion group,the rats were treated with intravenous RES 20 mg·kg^-1 in every rat and normal saline imme-diately after the occlusion of superior mesenteric artery（SMA）.The SMA was clamped for 45 minutes prior to reperfusion.Specimens were obtained at 6 h after the model establishment.Blood samples were collected for detecting the activity of diamine oxidase（DAO）and small intestine fatty acid-binding protein（IFABP）.The apoptosis of the mucosal cells was detected by terminal-deoxynucleotidyl transferase mediated nick end labeling method.And the histological examination was performed by the method of staining with hematoxylin-eosin. Results After 6 h of reperfusion,the levels of DAO and IFABP in RES group were significantly decreased compared with those in ischemia-reperfusion group [DAO（1 650±1 150） U·L^-1 vs（2 920±1 520） U·L^-1;IFABP（845.12±123.86） μg·L^-1 vs（1 443.76±174.62） μg·L^-1,Pa0.05],but they were all significantly increased compared with those in sham-operation group[（630±150） U·L^-1,（26.76±4.86） μg·L^-1,P〈0.05].In sham-operation group,there were only a few scattered apoptotic cells at the top of intestinal villi,inherent layers and the submucosa.In ischenmia-reperfusion group,the number of apoptotic cells was significantly increased compared with that in sham-operation group[（66.63±1.71）% vs（9.60±1.76）%,P〈0.05],ranged from the top to the bottom of intestinal villi,inherent layers and the submucosa.While in RES group,the apoptotic cells[（46.72±1.50）%] was significantly decreased compared with that in ischemia-reperfusion group（P〈0.05）. Conclusions It suggests that RES has a protective effect on intestine against ischemia-reperfusion injury in rats.The mechanism of protection may be related to its inhibition on apoptosis of the intestinal mucosa cells.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第23期1784-1786,共3页
Journal of Applied Clinical Pediatrics
关键词
白藜芦醇
缺血再灌注
细胞凋亡
肠黏膜通透性
resveratrol
ischemia-reperfusion
cell apoptosis
intestinal mucosal permeability
