摘要
目的分析XRCC1 Codon399单核苷酸多态性与鼻咽癌患者正常组织晚期放射性损伤的相关性。方法经病理学检查确诊的60例鼻咽癌患者,放疗前提取全血基因组DNA,进行PCR扩增及LDP连接检测反应扩增,得出XRCC1 Codon399的3种基因型。随访至放疗结束后3个月,对晚期放射性损伤进行评价,比较XRCC1 Codon399不同基因型与正常组织晚期放射性损伤的相关性。结果 60例患者中,XRCC1 Codon399 Gln/Gln基因型6例,Arg/Gln基因型21例,Arg/Arg基因型33例,不同性别之间无明显差异。Gln/Gln基因型者1、2、3级皮肤晚期放射性损伤发生率分别为33.3%、66.7%、0,Arg/Gln基因型者为52.4%、42.9%、4.7%,Arg/Arg基因型者为63.6%、36.4%、0;Gln/Gln基因型患者1、2、3级皮下组织晚期放射性损伤发生率分别为33.3%、66.7%、0,Arg/Gln基因型者为38.1%、57.2%、4.7%,Arg/Arg基因型者为42.4%、57.6%、0;Gln/Gln基因型患者1、2、3级黏膜晚期放射性损伤发生率分别为100.0%、0、0,Arg/Gln基因型者分别为80.9%、14.3%4.7%,Arg/Arg基因型者分别为93.9%、6.1%、0;Gln/Gln基因型患者1、2、3级涎腺晚期放射性损伤发生率分别为100.0%、0、0,Arg/Gln基因型者分别为80.9%、14.3%、4.7%,Arg/Arg基因型者分别为93.9%、6.1%、0。经统计分析,不同基因型患者间晚期放射性损伤发生率无明显差异(P>0.05)。结论 XRCC1 Co-don399单核苷酸多态性与鼻咽癌患者皮肤、皮下组织、黏膜、涎腺晚期放射性损伤程度无明显相关性,尚不能将其应用于对鼻咽癌患者正常组织晚期放射性损伤程度的预测。
Objective By detecting the single nucleotide polymorphisms（SNPs） of XRCC1 Codon399 of peripheral blood of patients suffering from nasopharyngeal carcinoma（NPC）,the relationshipship between SNPs of XRCC1 Codon399 and radiation injury in normal tissues was analysed.Methods 60 patients with pathologically confirmed low differentiated squamous cell carcinoma of the nasopharynx were included in the study.Genome DNA was extracted from whole blood cells before radiotherapy and the PCR products were amplified by ligase detection reaction（LDR）.The LDR products were put on sequencer to carry out electrophoresis,get the genotype of XRCC1 Codon399,evaluate the clinical effect in three months after radiotherapy and compare the chronic radiation reactions of normal tissue in the different genotypes.Results Of the 60 cases,the number of XRCC1 Codon399 Gln/Gln,Arg/Gln and Arg/Arg was 6,21 and 33,respectively.The incidence of grade Ⅰ,Ⅱ and Ⅲ chronic irradiation-induced injury of skin was 33.3%,66.7% and 0 with Gln/Gln,52.4%,42.9% and 4.7% with Arg/Gln,63.6%,36.4% and 0 with Arg/Arg.The rate of grade Ⅰ,Ⅱ and Ⅲchronic irradiation-induced injury of subcutaneous tissues was 33.3%,66.7% and 0 with Gln/Gln;42.4%,57.6% and 0 with Arg/Gln;38.1%,57.2% and 4.7% with Arg/Arg genotype.The rate of grade Ⅰ,Ⅱ and Ⅲchronic irradiation-induced injury of mucosa was 100.0%,0,0 with Gln/Gln;93.9%,6.1%,0 with Arg/Gln;80.9%,14.3%,4.7% with Arg/Arg genotype respectively.The rate of the grade Ⅰ,Ⅱ and Ⅲchronic irradiation-induced injury of parotid gland was 100.0%,0,0 with Gln/Gln,93.9%,6.1%,0 with Arg/Gln,80.9%,14.3%,4.7% with Arg/Arg genotype respectively.There were no significant differences among the three genotypes（P0.05）.Conclusion There was no relationship between the SNPs of XRCC1 Codon399 and chronic irradiation-induced injury of normal tissues in NPC patients.
出处
《实用癌症杂志》
2011年第6期596-599,共4页
The Practical Journal of Cancer
关键词
XRCC1单核苷酸多态性
鼻咽癌
放射损伤
XRCC1 single nucleotide polymorphisms
Nasopharyngeal carcinoma
Chronic irradiation-induced injury
