摘要
目的研究高效液相色谱测定血清中维生素B1(VB1)和维生素B2(VB2)的方法。方法采用C18反相色谱柱,以甲醇+磷酸盐缓冲溶液(pH3.5)作为流动相;二极管阵列检测器检测;采用Sep-park C18小柱吸附的样品前处理方法,考察了保留时间,洗脱液体积和比例对样品前处理效果的影响。结果方法的回收率82.67%～96.89%,变异系数<10%;线性相关系数分别为0.9985和0.9995,线性关系良好。测定正常人和肥胖人血清中VB1和VB2的含量,结果显示肥胖人血清中两种维生素的含量显著低于正常人。结论建立了HPLC测定血清中VB1和VB2的方法,肥胖人体内缺乏VB1和VB2。
Objective To develop a HPLC method for the determination of serum vitamin B1（VB1）and vitamin B2（VB2）simultaneously.Methods Serum samples were pretreated at ambient temperature on a C18 reversed phase chromatographic column,methanol plus phosphate buffer was used for mobile phase,vitamin B1 and B2 contents of serum samples eluted from a Sep-park C18 column were detected with a photodiode array detector.The influences of retention time,the volume and the proportion of eluent on the recovery of samples were investigated.Results Recoveries were ranged from 82.67 to 96.89%.The coefficient of variation was 10%.The linearity and correlation coefficient was 0.9985 and 0.9995,respectively.The contents of serum VB1 and VB2 in obese people were lower than those in healthy controls.Conclusion A HPLC method of determining serum VB1 and VB2 was established.Serum VB1 and VB2 level in obese people was low.
出处
《卫生研究》
CAS
CSCD
北大核心
2011年第6期756-758,共3页
Journal of Hygiene Research
基金
国家自然科学基金中加合作项目(No.30811120432)
