摘要
【目的】建立大白菜真叶高效再生体系。【方法】选择‘06J28’、‘92S105’、‘06J30’、‘06J31’4种不同基因型大白菜作为试验材料,以刚长出第2片真叶的大白菜幼苗的第1片真叶为外植体,研究不同激素(6-BA、NAA、TDZ)组合、外植体切割与接种方式以及不同基因型对真叶不定芽再生的影响。【结果】在MS+4.0mg/L 6-BA+0.14mg/L NAA培养基上培养时,‘06J28’真叶不定芽再生率和再生系数达到最大分别为87.63%和2.62;在MS+0.3mg/L TDZ+0.14mg/L NAA培养基上培养时,‘06J28’真叶不定芽的再生率和再生系数分别为80.73%和1.89,表明TDZ的作用与6-BA相似。在真叶柄基部和下胚轴交接处横切,保留完整真叶作为外植体为最佳切割方式,以真叶柄的下端竖直向下插入培养基为最佳接种方式。在供试的4种基因型材料中,‘06J28’真叶的再生率最高,达87.63%;‘92S105’的再生系数最高,达3.76。【结论】建立了大白菜真叶高效再生体系,为大白菜转基因研究奠定了基础。
【Objective】 The study was done to establish a high regeneration system of heading Chinese cabbage.【Method】 The effects of different combinations of hormones（6-BA,NAA,TDZ）,cutting treatments and inoculation methods on regeneration rate of adventitious bud were studied in four heading Chinese cabbage with the first leaf as explants when the second leaf just came out.【Result】 The results showed that the best regeneration rate and regeneration coefficient of ＇06J28＇ reached as high as 87.63% and 2.62 respectively in MS＋4.0 mg/L 6-BA＋0.14 mg/L NAA;The regeneration rate and regeneration coefficient of ＇06J28＇ were 80.73% and 1.89 respectively in the MS medium supplemented with 0.3 mg/L TDZ and 0.14 mg/L NAA,and TDZ showed similar effect with 6-BA;The best cutting and inserting mode was that：The young leaf was cut off at the cross of petiol and hypocotyl,the whole leaf with petiole was kept as explant,which was vertically inserted in medium with petiol end;Comparing the four genotypes,the highest regeneration rate reached 87.63% in ＇06J28＇,while the highest regeneration coefficient reached 3.76 in ＇92S105＇.【Conclusion】 We established a high regeneration system of heading Chinese cabbage and set up a basis for transgenic improvement further.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2011年第11期146-152,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家科技支撑计划项目(2009BADB8B03-1)
农业科技成果转化资金项目(2010GB2G000462)
陕西省蔬菜产业体系
西北农林科技大学唐仲英育种基金项目
关键词
大白菜
真叶
离体培养
再生体系
heading Chinese cabbage
leaf
culture in vitro
regeneration system
