摘要
目的:探讨羽扇豆醇对人胰腺癌细胞SW1990及γδT细胞生长的影响.方法:采用本实验室体外扩增人外周血γδT细胞,用四甲基偶氮唑蓝(MTT)法检测不同浓度的羽扇豆醇在不同时间段对人γδT细胞和人胰腺癌细胞SW1990生长的影响.本实验分3组:空白对照组、溶剂对照组和实验组,时间段分24、48、72h组.结果:羽扇豆醇浓度在0.4-200μg/mL时羽扇豆醇对人胰腺癌细胞SW1990在24、48、72h各组与空白对照组和溶剂对照组相比差异有统计学意义(P<0.05).而24、48、72h3组比较差异无统计学意义.γδT细胞培养7d从扩增前的3.61%增加到80.2%,羽扇豆醇对γδT细胞在24、48、72h随浓度的增加先增值后抑制作用.24h与空白对照组和溶剂对照组比较差异无统计学意义.48、72h各组与空白对照组和溶剂对照组相比差异有统计学意义(P<0.05).结论:羽扇豆醇对人胰腺癌细胞SW1990有抑制作用,对γδT细胞随着浓度增加有先促进后抑制作用.
AIM：To explore the effect of lupeol on the growth of human pancreatic cancer SW1990 cells andγδT cells.METHODS：Peripheral blood γδT cells,expandedin vitro,were used in our study.After SW1990and γδT cells were incubated with different concentrations of lupeol for different durations（24,48 and 72 h）,cell growth was determined byMTT assay.A blank control and a solvent control were run simultaneously.RESULTS：The Lupeol at doses of 0.4-200 μg/mL,there are significant differences of growth human pancreatic cancer cell line SW1990 cells be-tween the groups of 24,48,72 h and blank control group,the solvent control group（P 0.05）,however,no difference had been observed in each groups of three separated times.Harvested 7 d,γδT cells had expanded to 80.2% from 3.61%.in the groups of 24,48 and 72 h,the effects turned to be advantageous and then depressed as the dose was increasing.there are no differences between the groups of 24 h and blank control group,the solvent control group.there are significant differences between the groups of 48,72 h and blank control group,the solvent control group（P 0.05）.CONCLUSION：Lupeol suppressed the growth of SW1990 cells,but produced a facilitative effect first and then inhibitory effect on the growth of γδT cells.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第28期2958-2962,共5页
World Chinese Journal of Digestology
